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BUV395 Rat Anti-Mouse Vβ 6 T-Cell Receptor
Product Details
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BD OptiBuild™
TCR V beta 6; TCR Vβ6
Mouse (Tested in Development)
Rat F344, also known as Fischer, CDF IgG2b, λ
C57BL/6 Mouse Helper T-Cell Clone OI11
Flow cytometry (Qualified)
0.2 mg/ml
110317
AB_2742345
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
744596 Rev. 1
Antibody Details
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RR4-7

The RR4-7 antibody specifically reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL) and b (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the Tcrb gene complex. The Tcrb-V6 gene locus is deleted in mice having the c (e.g., RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by Mtv-7 (Mls-1[a], Mls[a]) endogenous provirus (e.g., AKR, CBA/J, C58, DBA/2, NZB), or Mtv-43 endogenous provirus (e.g., MA/MyJ). Exogenous MMTV-SW, as well as endogenous Mtv-44-encoded superantigen (e.g., NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks in vitro proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

744596 Rev. 1
Format Details
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BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV395
Ultraviolet 355 nm
348 nm
395 nm
744596 Rev.1
Citations & References
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View product citations for antibody "744596" on CiteAb

Development References (14)

  1. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology). View Reference
  2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
  3. Held W, Shakhov AN, Waanders G, et al. An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7). J Exp Med. 1992; 175(6):1623-1633. (Biology). View Reference
  4. Jones LA, Chin LT, Longo DL, Kruisbeek AM. Peripheral clonal elimination of functional T cells. Science. 1990; 250(4988):1726-1729. (Biology). View Reference
  5. Jones LA, Chin LT, Merriam GR, Nelson LM, Kruisbeck AM. Failure of clonal deletion in neonatally thymectomized mice: tolerance is preserved through clonal anergy. J Exp Med. 1990; 172(5):1277-1285. (Biology). View Reference
  6. Kanagawa O, Palmer E, Bill J. The T cell receptor V beta 6 domain imparts reactivity to the Mls-1a antigen. Cell Immunol. 1989; 119(2):412-426. (Immunogen). View Reference
  7. Kanagawa O. In vivo T cell tumor therapy with monoclonal antibody directed to the V beta chain of T cell antigen receptor. J Exp Med. 1989; 170(5):1513-1519. (Clone-specific). View Reference
  8. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific). View Reference
  9. Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology). View Reference
  10. Rocha B, Vassalli P, Guy-Grand D. The V beta repertoire of mouse gut homodimeric alpha CD8+ intraepithelial T cell receptor alpha/beta + lymphocytes reveals a major extrathymic pathway of T cell differentiation. J Exp Med. 1991; 173(2):483-486. (Biology). View Reference
  11. Rudy CK, Kraus E, Palmer E, Huber BT. Mls-1-like superantigen in the MA/MyJ mouse is encoded by a new mammary tumor provirus that is distinct from Mtv-7. J Exp Med. 1992; 175(6):1613-1621. (Biology). View Reference
  12. Tomonari K, Fairchild S. Positive and negative selection of Tcrb-V6+ T cells. Immunogenetics. 1992; 36(4):230-237. (Biology). View Reference
  13. Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991; 21(4):1007-1011. (Clone-specific). View Reference
  14. Webb S, Morris C, Sprent J. Extrathymic tolerance of mature T cells: clonal elimination as a consequence of immunity. Cell. 1990; 63(6):1249-1256. (Biology). View Reference
View All (14) View Less
744596 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.