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BUV395 Mouse Anti-Human IL-9 Receptor Alpha (CD129)
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Product Details
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BD OptiBuild™
CD129; Interleukin-9 receptor; IL-9R alpha chain; IL-9Rα
Human (Tested in Development)
Mouse IgG2b, κ
Human IL9R Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2743125
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
745622 Rev. 1
Antibody Details
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AH9R7

The AH9R7 monoclonal antibody specifically binds to the Interleukin-9 Receptor (IL-9R, IL-9Rα) also known as CD129. CD129 is a Type I transmembrane glycoprotein and a Type I Cytokine Receptor (Hemopoietin) Superfamily member. The high-affinity, signaling IL-9 Receptor complex is comprised of CD129 (IL-9 Receptor alpha chain subunit) and CD132 (common γ chain/γc).  CD129 is expressed by hematopoietic progenitors, thymocytes, T cells, B cells, macrophages, eosinophils, mast cells, epithelia cells, muscle cells and neurons.  IL-9 signals through the IL-9R complex (ie, through JAK, STAT and related signal pathways) to regulate the growth, proliferation, differentiation and survival of myeloid and erythroid progenitor cells, thymocytes, T cells, B cells and some tumor cells.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

745622 Rev. 1
Format Details
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BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV395
Ultraviolet 355 nm
348 nm
395 nm
745622 Rev.1
Citations & References
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View product citations for antibody "745622" on CiteAb

Development References (6)

  1. De Smedt M, Verhasselt B, Kerre T, et al. Signals from the IL-9 receptor are critical for the early stages of human intrathymic T cell development. J Immunol. 2000; 164(4):1761-1767. (Clone-specific: Inhibition). View Reference
  2. Demoulin JB, Uyttenhove C, Van Roost E, et al. A single tyrosine of the interleukin-9 (IL-9) receptor is required for STAT activation, antiapoptotic activity, and growth regulation by IL-9. Mol Cell Biol. 1996; 16(9):4710-4716. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
  3. Grasso L, Huang M, Sullivan CD, et al. Molecular analysis of human interleukin-9 receptor transcripts in peripheral blood mononuclear cells. Identification of a splice variant encoding for a nonfunctional cell surface receptor. J Biol Chem. 1998; 273(37):24016-24024. (Clone-specific: Flow cytometry, IC/FCM Block, Immunohistochemistry, Immunoprecipitation). View Reference
  4. Pilette C, Ouadrhiri Y, Van Snick J, et al. IL-9 inhibits oxidative burst and TNF-alpha release in lipopolysaccharide-stimulated human monocytes through TGF-beta. J Immunol. 2002; 168(8):4103-4111. (Clone-specific: Flow cytometry, Inhibition). View Reference
  5. Pilette C, Ouadrhiri Y, Van Snick J, et al. Oxidative burst in lipopolysaccharide-activated human alveolar macrophages is inhibited by interleukin-9. Eur Respir J. 2002; 20:1198-1205. (Clone-specific: Functional assay, Inhibition). View Reference
  6. Wilhelm C, Turner JE, Van Snick J, Stockinger B. The many lives of IL-9: a question of survival?. Nat Immunol. 2012; 13(7):637-641. (Biology). View Reference
View All (6) View Less
745622 Rev. 1

 

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.