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APC Mouse Anti-Human CD41a
APC Mouse Anti-Human CD41a

Flow cytometric analysis of CD41a expression on human peripheral blood platelets. Platelets were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Mouse Anti-Human CD41a (Cat. No. 559777/561852; solid line histogram). Fluorescent histograms depicting CD41a (or Ig isotype) expression were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™.

Flow cytometric analysis of CD41a expression on human peripheral blood platelets. Platelets were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Mouse Anti-Human CD41a (Cat. No. 559777/561852; solid line histogram). Fluorescent histograms depicting CD41a (or Ig isotype) expression were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™.

Product Details
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BD Pharmingen™
ITGA2B; Integrin alpha-2b (αIIb); Platelet glycoprotein IIb (GPIIb)
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Mouse BALB/c IgG1, κ
Purified platelet membrane glycoproteins
Flow cytometry (Routinely Tested)
20 µl
IV P38
3674
AB_398671
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559777 Rev. 8
Antibody Details
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HIP8

The HIP8 monoclonal antibody specifically binds to the α-chain of CD41. CD41 is also known as Integrin αIIb or Platelet GPIIb. The calcium-dependent complex of CD41 and CD61 (β3 integrin or GPIIIa) is normally expressed on platelets and megakaryocytes. The CD41/CD61 complex is the receptor for fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation. CD41 (clone HIP8) completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.

Clone HIP8 also cross-reacts with a major subset of peripheral blood platelets of baboon, and both rhesus and cynomolgus macaque monkeys. The staining pattern of platelets is similar to that observed with peripheral blood platelets from normal human donors.

559777 Rev. 8
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
559777 Rev.8
Citations & References
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Development References (2)

  1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
559777 Rev. 8

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.