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BD Pharmingen™ APC Mouse anti-Human CD39
Clone TU66 (also known as Tü 66, Tü66) (RUO)
Flow cytometric analysis of APC anti-human CD39 on peripheral blood. Human peripheral blood was stained simultaneously with FITC anti-human CD4 (clone RPA-T4, Cat. No. 5555346), PE-Cy7 anti-human CD25 (Clone M-A251, Cat. No. 557741), PE anti-human CD127 (clone hIL-7R-M21, Cat No. 557938) and APC anti-human CD39 (clone TU66) or an APC conjugated mouse IgG2b, κ isotype control (clone 27-35, Cat. No. 555745). Cells were then lysed and CD39 expression examined. CD39 expression is shown on regulatory T cells (solid line) versus isotype control (dotted line,left panel). RegulatoryT cells were identified from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ cells shown as CD25bright,CD127dim population. (right panel). Flow cytometry was performed on a BD FACSCanto™ System.
Flow cytometric analysis of APC anti-human CD39 on peripheral blood. Human peripheral blood was stained simultaneously with FITC anti-human CD4 (clone RPA-T4, Cat. No. 5555346), PE-Cy7 anti-human CD25 (Clone M-A251, Cat. No. 557741), PE anti-human CD127 (clone hIL-7R-M21, Cat No. 557938) and APC anti-human CD39 (clone TU66) or an APC conjugated mouse IgG2b, κ isotype control (clone 27-35, Cat. No. 555745). Cells were then lysed and CD39 expression examined. CD39 expression is shown on regulatory T cells (solid line) versus isotype control (dotted line,left panel). RegulatoryT cells were identified from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ cells shown as CD25bright,CD127dim population. (right panel). Flow cytometry was performed on a BD FACSCanto™ System.
Flow cytometric analysis of APC anti-human CD39 on peripheral blood. Human peripheral blood was stained simultaneously with FITC anti-human CD4 (clone RPA-T4, Cat. No. 5555346), PE-Cy7 anti-human CD25 (Clone M-A251, Cat. No. 557741), PE anti-human CD127 (clone hIL-7R-M21, Cat No. 557938) and APC anti-human CD39 (clone TU66) or an APC conjugated mouse IgG2b, κ isotype control (clone 27-35, Cat. No. 555745). Cells were then lysed and CD39 expression examined. CD39 expression is shown on regulatory T cells (solid line) versus isotype control (dotted line,left panel). RegulatoryT cells were identified from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ cells shown as CD25bright,CD127dim population. (right panel). Flow cytometry was performed on a BD FACSCanto™ System.
Flow cytometric analysis of APC anti-human CD39 on peripheral blood. Human peripheral blood was stained simultaneously with FITC anti-human CD4 (clone RPA-T4, Cat. No. 5555346), PE-Cy7 anti-human CD25 (Clone M-A251, Cat. No. 557741), PE anti-human CD127 (clone hIL-7R-M21, Cat No. 557938) and APC anti-human CD39 (clone TU66) or an APC conjugated mouse IgG2b, κ isotype control (clone 27-35, Cat. No. 555745). Cells were then lysed and CD39 expression examined. CD39 expression is shown on regulatory T cells (solid line) versus isotype control (dotted line,left panel). RegulatoryT cells were identified from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ cells shown as CD25bright,CD127dim population. (right panel). Flow cytometry was performed on a BD FACSCanto™ System.
Product Details
BD Pharmingen™
ENTPD1; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
Human (QC Testing)
Mouse IgG2b, κ
Flow cytometry (Routinely Tested)
20 µl
IV A54
AB_1645459
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
APC Mouse IgG2b κ Isotype Control RUO
Size
100 Tests
Cat No.
555745
PE Mouse Anti-Human CD127 RUO
Size
0.1 mg
Cat No.
557938
FITC Mouse Anti-Human CD4 RUO
Size
100 Tests
Cat No.
555346
PE-Cy™7 Mouse Anti-Human CD25 RUO
Size
100 Tests
Cat No.
557741
560239 Rev. 1
Antibody Details
TU66
The TU66 monoclonal antibody specifically recognizes human CD39 which is also known as Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), Ecto-ATP diphosphohydrolase 1 (Ecto-ATPDase 1), or Ecto-apyrase. CD39 is an integral membrane glycoprotein with two transmembrane domains, N- and C-terminal cytoplasmic tails, and an extracellular region that contains the NTPDase 1 active site. CD39 is encoded by ENTPD1 which belongs to the ectoenzyme family. CD39 is variably expressed on activated T cells and B cells, regulatory T cells (Treg), dendritic cells, Langerhans cells, NK cells, monocytes, macrophages, endothelial cells, and granulocytes. CD39 acts on extracellular nucleoside triphosphates and diphosphates including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.
560239 Rev. 1
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
651 nm
660 nm
560239 Rev.1
Citations & References
View product citations for antibody "560239" on CiteAb
Development References (4)
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Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.. Blood. 2007. (Biology). View Reference
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Duensing S, Kirshner H, Atzpodien J. CD39 as a novel marker of in vivo immune activation. Blood. 1994; 83(12):3826-3827. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
560239 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.