BD Pharmingen™ NHP T Lymphocyte Cocktail

The NHP T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify NHP T lymphocytes by direct immunofluorescence staining with flow cytometric analysis.  The SK1 antibody reacts with the hinge-like membrane-proximal domain of the 32-kDa alpha chain of the CD8 differentiation antigen..  The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells).  The L200 antibody reacts with the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. L200 antibody also cross-reacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both rhesus and cynomolgus macaque monkeys. Cross-reactivity on both lymphocytes and monocytes (weak) of baboon is also observed. The distribution on lymphocytes is similar for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.  The SP34-2 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes and peripheral T lymphocytes.  Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys.