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This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining. This kit also provides an alternative protein transport inhibitor, BD GolgiPlug™ containing brefeldin A. Sufficient BD GolgiPlug reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10⁶ cells/ml.
Components Quantity Fixation/Permeabilization solution 125 mL BD Perm/Wash™ buffer (10x) 100 mL BD GolgiPlug protein transport inhibitor containing brefeldin A 1 mL
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| N/A | 1.0 | N/A | N/A |
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
