PE Mouse Anti-Human glycophorin A

BD™ PE Mouse Anti-Human glycophorin A

Name: PE Mouse Anti-Human glycophorin A
Brand: BD™
SKU: 340947

Clone GA-R2 (HIR2)

(RUO (GMP))

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Alternative Name: CD235ab; CD235a, Glycophorin-A, GYPA, GPA; CD235b; Glycophorin-B, GYPB, GPB

Reactivity: Human

Isotype: Mouse IgG2b, κ

Application: Flow cytometry

Concentration: 25 µg/mL

Vol. Per Test: 20 μL

Workshop Number: VII 70299

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The PE conjugate is supplied as 25 µg in 1.0 mL (25 µg/mL) of phosphate-buffered saline (PBS). PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340947 Rev. 1

Antibody Details

GA-R2 (HIR2)

GAR-2(HIR-2)

Anti–glycophorin A reacts with a major sialoglycoprotein present on the human erythrocyte membrane. Glycophorin A is a transmembrane dimeric complex of 31 kilodaltons (kd) with its caboxyterminal ends extending into the cytoplasm of the red cell.

340947 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340947 Rev.1

Citations & References

View product citations for antibody "340947" on CiteAb

Development References (11)

Bain B, Catovsky D. Current concerns in haematology 2: classification of acute leukaemia. J Clin Pathol. 1990; 43:882-887. (Biology).
Bain BJ. Leukaemia Diagnosis: A Guide to the FAB Classiﬁcation. London: Gower Medical Publishing; 1990:65-68.
Gahmberg CG, Jokinen M, Andersson LC. Expression of the major sialoglycoprotein (glycophorin) on erythroid cells in human bone marrow. Blood. 1978; 52:379-387. (Biology).
Helleberg C, Knudsen H, Hansen PB, et al. CD34+ megakaryoblastic leukaemic cells are CD38-, but CD61+ and glycophorin A+: improved criteria for diagnosis of AML-M7? . Leukemia. 1997; 830-834. (Biology).
Jokinen M, Ehnholm C, Väisänen-Rhen V, et al. Identification of the major human sialoglycoprotein from red cells, glycophorin AM, as the receptor for Escherichia coli IH 11165 and characterization of the receptor site. Eur J Biochem. 1985; 147:47-52. (Biology).
Loken MR, Civin CI, Bigbee WL, Langlois RG, Jensen RH. Coordinate glycosylation and cell surface expression of glycophorin A during normal human erythropoiesis. Blood. 1987; 70(6):1959-1961. (Biology). View Reference
Rogers CE, Bradley MS, Palsson BO, Koller MR. Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship with burst-forming units-erythroid. Exp Hematol. 1996; 24(5):597-604. (Biology). View Reference
Templeton TJ, Keister DB, Muratova O, Procter JL, Kaslow DC. Adherence of erythrocytes during exflagellation of Plasmodium falciparum microgametes is dependent on erythrocyte surface sialic acid and glycophorins. J Exp Med. 1998; 187(10):1599-1609. (Biology). View Reference
Wise GE, Oakford LX, Dzandu JK. Ultrastructure of a transmembrane glycoprotein, glycophorin A. Tissue & Cell. 1988; 20:219-227. (Biology).
Wybenga LE, Epand RF, Nir S, et al. Glycophorin as a receptor for sendai virus. Biochem. 1996; 35:9513-9518. (Biology).
de Vries E, De Bruin-Versteeg S, Comans-Bitter WM, et al. Correction for erythroid cell contamination in microassay for immunophenotyping of neonatal lymphocytes. Arch Dis Child Fetal Neonatal Ed. 1999; 80:226-229. (Biology).

View All (11)

340947 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.