FITC Mouse Anti-Human CD161

BD™ FITC Mouse Anti-Human CD161

Name: FITC Mouse Anti-Human CD161
Brand: BD™
SKU: 340536

Clone DX12

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: KLRB1; CLEC5B; NKR; NKR-P1A: HNKR-P1a

Reactivity: Human

Isotype: Mouse C3H, also known as C3H/He, C3H/Bi IgG1, κ

Immunogen: Human NKR-P1A Transfected Mouse Fibroblasts

Application: Flow cytometry

Vol. Per Test: 20 μL

Workshop Number: VI NK12

Entrez Gene ID: 3820

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The FITC and PE conjugates are each supplied in 1.0 mL of PBS. PBS contains gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340536 Rev. 1

Antibody Details

DX12

CD161, clone DX12, is derived from splenocytes from C3H/HeJ mice immunized with murine fibroblasts stably transfected with hNKR-P1A cDNA in the pBJ expression vector and fused with Sp2/0 myeloma cells.

CD161 recognizes the human NKR-P1A antigen, a disulfide-linked homodimer of a 40-kdalton (kDa) glycoprotein reduced to 26 kDa by treatment with N-glycanase.

340536 Rev. 1

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

340536 Rev.1

Citations & References

View product citations for antibody "340536" on CiteAb

Development References (3)

Bennett IM, Zatsepina O, Zamai L, Azzoni L, Mikheeva T, Perussia B. Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. J Exp Med. 1996; 184(5):1845-1856. (Biology). View Reference
Lanier LL, Chang C, Phillips JH. Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J Immunol. 1994; 153(6):2417-2428. (Biology). View Reference
Ryan JC, Niemi EC, Nakamura MC, Seaman WE. NKR-P1A is a target-specific receptor that activates natural killer cell cytotoxicity. J Exp Med. 1995; 181(5):1911-1915. (Biology). View Reference

340536 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.