AmCyan Mouse Anti-Human CD45

BD™ AmCyan Mouse Anti-Human CD45

Name: AmCyan Mouse Anti-Human CD45
Brand: BD™
SKU: 339192

Clone 2D1

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5

Reactivity: Human

Isotype: Mouse IgG1, κ

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 5 μL

Storage Buffer: Phosphate buffered saline with BSA and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

339192 Rev. 1

Antibody Details

2D1

The CD45 antibody, clone 2D1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The CD45 antibody (anti–HLe-1) recognizes members of the T200 family of human leucocyte antigens with molecular mass of 180 to 220 kilodaltons (kDa.)

339192 Rev. 1

Format Details

AmCyan

The AmCyan™ dye is part of the BD violet family of dyes. This dye is a fluorescent protein with an excitation maximum (Ex Max) of 457-nm and an emission maximum (Em Max) at 489 nm. AmCyan is designed to be excited by the Violet laser (405-nm) and detected using an optical filter centered near 500-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: AmCyan

339192 Rev.1

Citations & References

View product citations for antibody "339192" on CiteAb

Development References (8)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Hermiston ML, Xu Z, Weiss A. CD45: a critical regulator of signaling thresholds in immune cells. Annu Rev Immunol. 2003; 21:107-137. (Biology). View Reference
Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
Loken MR, Brosnan JM, Bach BA, Ault KA. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry. 1990; 11(4):453-459. (Biology). View Reference
Pellat-Deceunynck C, Bataille R. Normal and malignant human plasma cells: proliferation, differentiation, and expansions in relation to CD45 expression. Blood Cells Mol Dis. 2004; 32:293–301. (Biology).
Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
Terstappen LW, Levin J. Bone marrow cell differential counts obtained by multidimensional flow cytometry. Blood Cells. 1992; 18(2):311-330. (Biology). View Reference

View All (8)

339192 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.