BD FastImmune™ Anti-Human CD8 FITC/CD69 PE/CD3 PerCP
Description
The CD8 antibody, clone SK1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. The CD69 antibody, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line. The CD3 antibody, clone SK7, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. The CD8 (Leu-2a) antibody recognizes the 32-kilodalton (kDa) α subunit of a disulfide-linked bimolecular complex of the CD8 antigen. The cytoplasmic domain of the α subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck. The CD8 molecule interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion. Binding of the CD8 molecule to class I MHC molecules enhances the activation of resting T lymphocytes. The CD69 antibody recognizes a very early human lymphocyte activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges. The CD3 antibody recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| N/A | N/A | | IgG1, κ,IgG1, κ,IgG1, κ | N/A |
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