R718 Rat Anti-Mouse CD107a (LAMP-1)
Flow cytometric analysis of CD107a expression on mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed line histogram) or BD Horizon™ R718 Rat Anti-Mouse CD107a antibody (Cat. No. 566986; solid line histogram) at 0.5 µg/test. A histogram showing CD107a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ R718 Rat Anti-Mouse CD107a (LAMP-1)
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