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PE Mouse Anti- IRF4
PE Mouse Anti- IRF4
Flow cytometric analysis of IRF4 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells (PBMC) were not stimulated (Top Left Plot) or stimulated (Top Right Plot) with phytohemagglutinin (PHA; 3 days). C57BL/6 mouse splenic B cells were not stimulated (Bottom Left Plot) or stimulated (Bottom Right Plot) with lipopolysaccharide (LPS; 2 days). PBMC were then fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-IRF4 antibody (Cat. No. Cat. No. 566646/566649; solid line histograms) at 0.25 µg/test. Mouse cells were similarly fixed, permeabilized, and stained using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). Histograms showing IRF4 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of IRF4 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells (PBMC) were not stimulated (Top Left Plot) or stimulated (Top Right Plot) with phytohemagglutinin (PHA; 3 days). C57BL/6 mouse splenic B cells were not stimulated (Bottom Left Plot) or stimulated (Bottom Right Plot) with lipopolysaccharide (LPS; 2 days). PBMC were then fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-IRF4 antibody (Cat. No. Cat. No. 566646/566649; solid line histograms) at 0.25 µg/test. Mouse cells were similarly fixed, permeabilized, and stained using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). Histograms showing IRF4 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
IRF4; IRF-4; interferon regulatory factor 4; LSIRF; MUM1; NF-EM5; SHEP8
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1, κ
Mouse IRF4 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869805
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566649 Rev. 1
Antibody Details
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Q9-343

The Q9-343 monoclonal antibody specifically recognizes human and mouse Interferon Regulatory Factor 4 (IRF4 or IRF-4) which is also known as Lymphocyte specific interferon regulatory factor (LSIRF), Multiple myeloma oncogene 1 (MUM1), or PU.1 interaction partner (PIP). IRF4 belongs to the Interferon Regulatory Factor (IRF) family of transcription factors that includes nine members, IRF1-9. IRF4 has a conserved N-terminal DNA binding domain with a unique tryptophan pentad repeat. Its C-terminal regulatory domain regulates IRF4 activity and mediates interactions with other IRF proteins, transcription factors and co-factors. IRF4 plays essential roles in the regulation of innate and adaptive immune responses. IRF4 is widely expressed in leucocytes and is essential for the development, activation, differentiation, and/or apoptosis of T helper (Th) cell subsets including Th2, Th9, Th17, T follicular helper (Tfh) cells, or T (Treg) cells. IRF4 is involved in the development or differentiation of CD8+ effector and memory cells, B cells and plasma cells, as well as different dendritic cell (DC) subsets and M2 macrophages. IRF4 is also expressed by adipocytes and melanocytes. Cellular IRF4 expression is primarily upregulated by antigen-receptor engagement, or by stimulation with lipopolysaccharide (LPS), CD40, or IL-4, rather than by interferons. TLR4 has been implicated in suppressing or promoting oncogenisis and autoimmunity.

        

566649 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566649 Rev.1
Citations & References
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Development References (6)

  1. Huber M, Lohoff M. IRF4 at the crossroads of effector T-cell fate decision.. Eur J Immunol. 2014; 44(7):1886-95. (Biology). View Reference
  2. Nam S, Lim JS. Essential role of interferon regulatory factor 4 (IRF4) in immune cell development.. Arch Pharm Res. 2016; 39(11):1548-1555. (Biology). View Reference
  3. Pernis AB. The role of IRF-4 in B and T cell activation and differentiation.. J Interferon Cytokine Res. 2002; 22(1):111-20. (Biology). View Reference
  4. Shaffer AL, Emre NC, Romesser PB, Staudt LM. IRF4: Immunity. Malignancy! Therapy?. Clin Cancer Res. 2009; 15(9):2954-61. (Biology). View Reference
  5. Yanai H, Negishi H, Taniguchi T. The IRF family of transcription factors: Inception, impact and implications in oncogenesis.. Oncoimmunology. 2012; 1(8):1376-1386. (Biology). View Reference
  6. Zhao GN, Jiang DS, Li H. Interferon regulatory factors: at the crossroads of immunity, metabolism, and disease.. Biochim Biophys Acta. 2015; 1852(2):365-78. (Biology). View Reference
View All (6) View Less
566649 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.