BV786 Rat Anti-Mouse CD119

If you're planning to order more than 10 BD OptiBuild™ Reagents, please contact your local sales representative to place this order. Contact Us

BD OptiBuild™ BV786 Rat Anti-Mouse CD119

Name: BV786 Rat Anti-Mouse CD119
Brand: BD OptiBuild™
SKU: 740897

Clone GR20

(RUO)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD OptiBuild™

Alternative Name: Ifngr1; IFN-γ Receptor α; IFN-gamma R alpha; Ifngr; INF-g Receptor; Cd119

Reactivity: Mouse (Tested in Development)

Isotype: Rat BN, also known as Brown Norway IgG2a, κ

Immunogen: BALB/c mouse monomyelocytic cell line WEHI-3

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Entrez Gene ID: 15979

RRID: AB_2740545

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
Cy is a trademark of Amersham Biosciences Limited.

Companion Products

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Lysing Buffer RUO

Size 100 mL Cat No. 555899

BV786 Rat IgG2a, κ Isotype Control RUO

Size 50 µg Cat No. 563335

View Details

740897 Rev. 1

Antibody Details

GR20

The GR20 monoclonal antibody specifically binds to CD119, the α chain of the interferon γ (IFN-γ) receptor. This receptor is expressed by mouse leukocytes, endothelial, and epithelial cells, but not by mature erythrocytes. GR20 mAb recognizes an epitope (susceptible to certain fixatives) in the ligand-binding site of the receptor. It can block the binding of mouse IFN-γ; therefore, it can inhibit IFN-γ .mediated effects including the activation of mouse macrophages for tumor cell killing. IFN-γ has been reported to induce the expression of Ly-6A/E antigen on cell lines and normal B lymphocytes, and the GR20 antibody is capable of inhibiting the IFN-γ.induced expression of Ly-6A/E on the mouse B lymphoma line A20. The binding of the GR20 antibody to CD119 is inhibited by IFN-γ. This antibody reportedly does not have any agonist effect.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

740897 Rev. 1

Format Details

BV786

The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm. BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.

Format: BV786

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 786 nm

740897 Rev.1

Citations & References

View product citations for antibody "740897" on CiteAb

Development References (4)

Basu M, Pace JL, Pinson DM, Hayes MP, Trotta PP, Russell SW. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc Natl Acad Sci U S A. 1988; 85(17):6282-6286. (Immunogen). View Reference
Codias EK, Malek TR. Regulation of B lymphocyte responses to IL-4 and IFN-gamma by activation through Ly-6A/E molecules. J Immunol. 1990; 144(6):2197-2204. (Biology). View Reference
Cofano F, Moore SK, Tanaka S, Yuhki N, Landolfo S, Appella E. Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptor. J Biol Chem. 1990; 265(7):4064-4071. (Biology). View Reference
LeClaire RD, Basu M, Pinson DM, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor.. J Leukoc Biol. 1992; 51(5):507-16. (Biology). View Reference

View All (4)

740897 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.