The 218820 monoclonal antibody specifically recognizes CD369 which is also known as Dendritic cell-associated C-type lectin 1 (DC-associated C-type lectin 1, Dectin-1, or Dectin1), or Beta-glucan receptor (Bgr). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7 member a). CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a cytoplasmic tail with an immunoreceptor tyrosine-based activation motif (ITAM). It is expressed on monocytes/macrophages, neutrophils, and some populations of dendritic cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 (Clec7a)-mediated signaling may play roles in leucocyte responses including phagocytosis or enhanced cytokine production.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).