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Intended as an aid in the diagnosis of hematological disorders



The BD OneFlow™ PCST is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1*


The BD OneFlow™ PCST is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow as an aid in the diagnosis of hematological disorders.


Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ Lymphoid Screening Tube (LST) , BD OneFlow™ B-cell Chronic Lymphoproliferative Diseases Tube 1 (B-CLPD T1)BD OneFlow™ Plasma Cell Disorders (PCD) Tube and BD OneFlow™ Acute Leukemia Orientation Tube (ALOT).


View video describing the BD OneFlow™ Solution on the BD FACSLyric™ Flow Cytometer.



The BD OneFlow™ PCST reagent composition


AntibodyFluorochromeCloneTubeTarget Populations
CD38FITCHB7SBackbone markers. Identification of plasma cells


PEMY31SIdentification of aberrant plasma cells
β2-MicroglobulinPerCP-Cy 5.5TU99SIdentification of aberrant plasma cells
CD19PE-CY 7 SJ25-C1SBackbone markers. Identification of plasma cells. Identify and characterize mature B cells
Anti-KappaAPCTB28-2CPlasma cells clonality
Anti-LambdaAPC-H71-155-2CPlasma cells clonality
CD45BD Horizon™ V4502D1SBackbone markers. Identification of plasma cells
CD138BD Horizon™ V500-CMI15SBackbone marker. Identification of plasma cells


A full description of the utility of the antibodies chosen for the BD OneFlow™ PCST is provided in the publication by the EuroFlow Consortium.1 






  1. van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120

  2. Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779

  3. Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122

BD Flow Cytometers are Class 1 Laser Products.

The EuroFlow trademark is the property of the EuroFlow Consortium and cannot be reproduced or published without prior written permission from the EuroFlow coordinator (

Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

The BD OneFlow™ Solution is intended for professional use only.

*This publication refers to EuroFlow and is relevant to the BD OneFlow™ Solution as the BD OneFlow™ Solution demonstrated equivalent results to EuroFlow through a clinical trial (BD OneFlow™ PCST Instructions for Use).