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Game 1: Resolution
Welcome to the BD Biosciences Fluorochrome Faceoff, where we explore the performance of various flow cytometry dyes excited primarily by the blue and yellow-green lasers!
This game will evaluate fluorochromes based on resolution (stain index).
What is Fluorochrome Resolution?
Fluorochrome resolution is the degree of separation between the negative and positive populations as calculated by Stain Index, (MFIpos-MFIneg)/2(rSDneg). CD4 is commonly used as a reference for Stain Index because of its robust expression and regular availability of the same clone in different formats. Here we will measure CD4 (SK3) Stain Index for several fluorochromes excited by the blue-laser with a peak emission around 780 nm.
Who Do You Think Will Win?
Competitors | |
BD Horizon RealBlue™ 780 (RB780) | BD Pharmingen™ PE-Cy7 |
BioLegend PerCP/Fire™ 780 | Cytek cFluor™ BYG781 (cFBYG781) |
*Note: Poll results do not determine the winners. Come back on September 12 to see game results and winners based on fluorochrome performance data.
Current Poll Standings as of September 4, 2024
RB780: 69% | PerCP/Fire™ 780: 8% | PE-Cy7: 15% | cFBYG781: 8%
The Results will be Announced Tuesday, September 10
Disclaimer: Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.
BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.