Anti-mouse Ig HRP Detection kit
- Brand BD Pharmingen™
- Regulatory Status RUO
Regulatory Status Legend
The BD Pharmingen™ Anti-Ig HRP Detection Kits are designed for immuno-histochemical staining of acetone-fixed frozen sections, formalin- or zinc-fixed paraffin sections, and cytospins/smears. The detection kit can be used with any purified primary antibody. Each kit includes a biotinylated anti-Ig antibody that is species-specific (anti-mouse, anti-rat or anti-hamster), antibody diluent, pre-diluted Streptavidin-horseradish peroxidase (SAv-HRP), and DAB substrate. The affinity-purified, polyclonal, biotinylated secondary antibody is adsorbed against multiple sera of related species, reducing non-specific staining, while providing maximum reactivity. Antibody diluent is a buffered solution containing protein stabilizers to enhance antibody reactivity, detergent for permeabilization, and blocking agents to reduce non-specific staining. The ultra-sensitive SAv-HRP is pre-diluted for convenience. DAB substrate is provided for signal development. Consistent results are assured with all these matched reagents. A comprehensive series of protocols, for immunostaining in different types of tissues, is provided with the kit.
Contents Quantity Goat anti-mouse Igs, biotinylated 0.5 mL Antibody Diluent Buffer 50 mL Streptavidin-HRP 20 mL DAB Buffer 20 mL DAB Chromogen 1.0 mL
Preparation and Storage
All the components of the Anti-Ig HRP Detection Kit must be stored at 4°C. Some solutions require diluting or mixing prior to use.