Measurement of the antigen expression of human bone marrow cells
Seven-laser excitation of seven fluorochromes is shown (right), demonstrating the utility of a seven-laser special order BD® LSR II Analyzer.*
Each spatially separated beam provides discrete fluorochrome excitation so that virtually no computer compensation is required for complete cell population separation. Data courtesy of Frederic Preffer (Harvard Medical School, Boston MA). Reference: Preffer F, Dombkowski D. Advances in complex multiparameter flow cytometry: Applications in stem cell research. Cytometry B Clin Cytom. 2009;76(5):295-314. doi: 10.1002/cyto.b.20480
*The BD® LSR product family includes the special order BD® LSR II and BD LSRFortessa™ Analyzers. This experiment was run on a BD® LSR II Analyzer configured with the following lasers: 20-mW 355-nm UV, 50-mW 405-nm violet, 20–100 mW 488-nm blue (variable power), 150-mW 532-nm green (variable power), 50-mW 594-nm yellow, 40-mW 638-nm red, and 25-mW 785-nm IR.