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BD Transduction Laboratories™ Purified Mouse Anti-ROCK-II
Clone 21/ROCK-II (RUO)
Western blot analysis of ROCK-II on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of ROCK-II.
MCF7
Western blot analysis of ROCK-II on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of ROCK-II.
MCF7
Product Details
BD Transduction Laboratories™
ROKα
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Rat ROKα aa. 567-718
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
180 kDa
250 µg/ml
AB_397956
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610624 Rev. 1
Antibody Details
21/ROCK-II
Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKα (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKα localizes at the cellular margins and colocalizes with acti filaments. The ROKα gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKα binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKα.
610624 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610624 Rev.1
Citations & References
View product citations for antibody "610624" on CiteAb
Development References (5)
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Adachi T, Vita R, Sannohe S, et al. The functional role of rho and rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils. J Immunol. 2001; 167(8):4609-4615. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Begum N, Sandu OA, Ito M, Lohmann SM, Smolenski A. Active Rho kinase (ROK-alpha) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells. J Biol Chem. 2002; 277(8):6214-6222. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Leung T, Manser E, Tan L, Lim L. A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes. J Biol Chem. 1995; 270(49):29051-29054. (Biology). View Reference
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Sahai E, Olson MF, Marshall CJ. Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility. EMBO J. 2001; 20(4):755-766. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Wang H, Eto M, Steers WD, Somlyo AP, Somlyo AV. RhoA-mediated Ca2+ sensitization in erectile function. J Biol Chem. 2002; 277(37):30614-30621. (Clone-specific: Western blot). View Reference
610624 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.