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Purified Mouse Anti-ERK1
Purified Mouse Anti-ERK1

Western blot analysis of ERK1. RSVtransformed mouse 3T3 fibroblasts were probed with anti-ERK1 (clone G262-118) at concentrations of 0.2 (lane 1), 0.04 (lane 2), and 0.008 µg/ml (lane 3). ERK1 is detected at ~44 kDa.

Western blot analysis of ERK1. RSVtransformed mouse 3T3 fibroblasts were probed with anti-ERK1 (clone G262-118) at concentrations of 0.2 (lane 1), 0.04 (lane 2), and 0.008 µg/ml (lane 3). ERK1 is detected at ~44 kDa.

Product Details
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BD Pharmingen™
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG2b
C-Terminal Region of ERK1
Western blot (Routinely Tested), Immunoprecipitation (Not Recommended)
44 kDa
0.5 mg/ml
AB_395238
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554100 Rev. 10
Antibody Details
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G262-118

A serine/threonine specific protein kinase, called MAP kinase or more recently ERK1 (extracellular signal regulated kinase) is activated in cells following stimulation with EGF (epidermal growth factor), the PDGF (platelet-derived factor) or insulin. ERK1 has been assayed in vitro for phosphotransferase activity using the microtubule-associated protein (MAP) or myelin basic protein. The mechanism by which MAP kinase becomes activated in growth factor treated cells is thought to involve tyrosine and threonine phosphorylation of the kinase itself. The EGF, PDGF and insulin receptors are known to be tyrosine specific protein kinases and studies have shown that MAP kinase is identical to the p42 protein that earlier studies identified as one of the major tyrosine phosphorylated proteins in transformed or growth factor treated cells. While several isoforms of the p42 protein are known, they are thought to be structurally similar. The G262-118 antibody recognizes human and mouse ERK1. It does not cross-react with ERK2. A synthetic peptide from the C-terminal region of ERK1 was used as immunogen.

554100 Rev. 10
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554100 Rev.10
Citations & References
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Development References (2)

  1. Campos-Gonzalez R, Glenney JR Jr. Temperature-dependent tyrosine phosphorylation of microtubule-associated protein kinase in epidermal growth factor-stimulated human fibroblasts. Cell Regul. 1993; 2(8):663-673. (Biology). View Reference
  2. Rossomando AJ, Payne DM, Weber MJ, Sturgill TW. Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase. Proc Natl Acad Sci U S A. 1989; 86(18):6940-6943. (Biology). View Reference
554100 Rev. 10

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.