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Purified Mouse Anti-Human MCL-1
Purified Mouse Anti-Human MCL-1
Western blot analysis of Mcl-1. Lysates from K-562 cells were probed with anti-human Mcl-1 antibody (clone 22, Cat. No. 559027) at a concentration of 2 µg/ml. Mcl-1 is identified at approximately 40 kDa.
Western blot analysis of Mcl-1. Lysates from K-562 cells were probed with anti-human Mcl-1 antibody (clone 22, Cat. No. 559027) at a concentration of 2 µg/ml. Mcl-1 is identified at approximately 40 kDa.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Purified recombinant human Mcl-1 lacking the C-terminal hydrophobic domain
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
40 kDa
0.5 mg/ml
AB_397176
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559027 Rev. 5
Antibody Details
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22/Mcl-1

The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.

559027 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559027 Rev.5
Citations & References
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View product citations for antibody "559027" on CiteAb

Development References (6)

  1. Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science. 1998; 281(5381):1322-1326. (Biology). View Reference
  2. Gross A, McDonnell JM, Korsmeyer SJ. BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 1999; 13(15):1899-1911. (Biology). View Reference
  3. Kozopas KM, Yang T, Buchan HL, Zhou P, Craig RW. MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc Natl Acad Sci U S A. 1993; 90(8):3516-3520. (Biology). View Reference
  4. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Biology). View Reference
  5. Wang JM, Chao JR, Chen W, Kuo ML, Yen JJ, Yang-Yen HF. The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB. Mol Cell Biol. 1999; 19(9):6195-6206. (Biology). View Reference
  6. Yang T, Kozopas KM, Craig RW. The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2. J Cell Biol. 1995; 128(6):1173-1184. (Biology). View Reference
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559027 Rev. 5

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.