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BD Transduction Laboratories™ Purified Mouse Anti-Human Lamp-1
Clone 25/Lamp-1 (RUO)
Western blot analysis of Lamp-1 on HepG2 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Lamp-1 antibody.
Western blot analysis of Lamp-1 on HepG2 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Lamp-1 antibody.
Product Details
BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2b
Human Lamp-1 aa. 25-224
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), Immunofluorescence (Not Recommended)
110 kDa
250 µg/ml
AB_398356
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611042 Rev. 1
Antibody Details
25/Lamp-1
Lysosomes are digestive compartments in mammalian cells that are responsible for the degradation of numerous substances including foreign materials. The lysosomal membrane is of interest due to its role in maintaining the acidic intralysosomal sac, its resistance to degradation, and its ability to fuse with other membranous organelles. Lamp-1 and lamp-2 (lysosomal-associated membrane proteins -1 and -2) were identified as lysosomal membrane proteins that act as carriers for poly-N-glycans, primarily N-acetyllactosamines. They consist of two heavily glycosylated amino terminal domains, a single trans-membrane domain, and a short cytoplasmic tail. A tyrosine located at the fourth residue from the end of the cytoplasmic tail, a large hydrophobic amino acid as the last residue, and a glycine residue amino terminal to the tyrosine residue targets these proteins from the TGN to the lysosome. The role of the Lamp molecules may be a carrier for poly-N-acetyllactosamines and/or to protect lysosomal membranes from the soluble hydrolases.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
611042 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611042 Rev.1
Citations & References
View product citations for antibody "611042" on CiteAb
Development References (5)
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Alto NM, Soderling J, Scott JD. Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics. J Biol Chem. 2002; 158(4):659-668. (Clone-specific: Immunofluorescence). View Reference
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Fukuda M, Viitala J, Matteson J, Carlsson SR. Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences. J Biol Chem. 1988; 263(35):18920-18928. (Biology). View Reference
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Fukuda M. Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking. J Biol Chem. 1991; 266(32):21327-21330. (Biology). View Reference
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Hocking DC, Kowalski K. A cryptic fragment from fibronectin's III1 module localizes to lipid rafts and stimulates cell growth and contractility. J Cell Biol. 2002; 158(1):175-184. (Clone-specific: Western blot). View Reference
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Spoerl Z, Stumpf M, Noegel AA, Hasse A. Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus. J Biol Chem. 2002; 277(50):48858-48867. (Clone-specific: Western blot). View Reference
611042 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.