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Purified Mouse Anti-Hsp70
Purified Mouse Anti-Hsp70

Western blot analysis of HSP-70 using

clone 5G10 (Cat. No 554253). HSP-70 was

constitutively expressed (lane 1) in HeLa

cells and increased approximately four fold

after a heat shock treatment at 42°C for 60

minutes (lane 2).

Western blot analysis of HSP-70 using

clone 5G10 (Cat. No 554253). HSP-70 was

constitutively expressed (lane 1) in HeLa

cells and increased approximately four fold

after a heat shock treatment at 42°C for 60

minutes (lane 2).

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2b
Purified Hsp70
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
70 kDa
0.5 mg/ml
AB_395322
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml. HeLa cervical carcinoma cells (ATCC CCL-2) are suggested as a positive control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554243 Rev. 8
Antibody Details
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5G10

Cells from many organisms increase the expression of heat shock or stress proteins in response to adverse changes. The heat shock protein (HSP) 70 family consists of multiple members, localized within different intracellular compartments. HSP 70 is constitutively expressed in many human cells with increased expression observed following stress. Members of the HSP 70 family bind ATP, and are thought to function as molecular chaperones by assisting in the folding of other proteins in various intracellular compartments. HSP 70 migrates at a molecular weight of 70 kDa in SDS-PAGE.

The 5G10 antibody recognizes human HSP 70. Purified HSP 70 was isolated from K562, a human erythroid cell line, and used as immunogen.

554243 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554243 Rev.8
Citations & References
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Development References (3)

  1. Craig EA, Gross CA. Is hsp70 the cellular thermometer. Trends Biochem Sci. 1991; 16(4):135-140. (Biology). View Reference
  2. Cyr DM, Langer T, Douglas MG. DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70. Trends Biochem Sci. 1994; 19(4):176-181. (Biology). View Reference
  3. Singh MK, Yu J. Accumulation of a heat shock-like protein during differentiation of human erythroid cell line K562. Nature. 1984; 309(5969):631-633. (Immunogen). View Reference
554243 Rev. 8

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.