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Purified Mouse Anti-RONα
Purified Mouse Anti-RONα

Western blot analysis of RONα on a MDCK cell lysate (Canine kidney; ATCC CCL-34). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RONα antibody.

Purified Mouse Anti-RONα

Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma; ATCC HTB-134).

Western blot analysis of RONα on a MDCK cell lysate (Canine kidney; ATCC CCL-34). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RONα antibody.

Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma; ATCC HTB-134).

Product Details
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BD Transduction Laboratories™
Recepteur d'Origine Nantaise
Dog (QC Testing), Human, Mouse, Rat, Frog (Tested in Development)
Mouse IgG2b
Human RONα aa. 40-224
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
40 kDa
250 µg/ml
AB_398068
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610744 Rev. 2
Antibody Details
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29/RONα

RON (Recepteur d'Origine Nantaise) is a tyrosine kinase receptor for MSP  (Macrophage Stimulating Protein) that belongs to the hepatocyte growth factor receptor family. RON is synthesized as a single chain glycosylated polypeptide of 1400 amino acids that is cleaved upon maturation into a 150 kDa β-subunit with the kinase activity and a 40 kDa α-chain. Both chains are linked by disulfide bonds and the proper arrangement of the α and β heterodimer is necessary for biological activity of RON. MSP induces the tyrosine kinase activity of RON, triggering a cascade of intracellular signaling that leads to DNA synthesis. However, RON activation in mouse erythroleukemia cells results in apoptosis. The replacement of autophosphorylation sites Y1330 and Y1337 abolishes both the mitogenic and apoptotic effects of MSP. Therefore, autophosphorylation at Y1330 and Y1337 is required for RON's biological activity. PLCγ, Shc, Grb2, p61, and p65 associate with RON. Although widely expressed, RON is particularly abundant in epithelial tissues, lung, brain, adrenal glands, gastrointestinal tract, kidney, and testis.

610744 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610744 Rev.2
Citations & References
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Development References (4)

  1. Gaudino G, Follenzi A, Naldini L. RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP. EMBO J. 1994; 13(15):3524-3532. (Biology). View Reference
  2. Peace BE, Hughes MJ, Degen SJ, Waltz SE. Point mutations and overexpression of Ron induce transformation, tumor formation, and metastasis. Oncogene. 2001; 20(43):6142-6151. (Biology: Immunoprecipitation, Western blot). View Reference
  3. Ronsin C, Muscatelli F, Mattei MG, Breathnach R. A novel putative receptor protein tyrosine kinase of the met family. Oncogene. 1993; 8(5):1195-11202. (Biology). View Reference
  4. Wang MH, Ronsin C, Gesnel MC, Coupey L. Identification of the ron gene product as the receptor for the human macrophage stimulating protein. Science. 1994; 266(5182):117-119. (Biology). View Reference
View All (4) View Less
610744 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.