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Purified Mouse Anti- PSD-95
Purified Mouse Anti- PSD-95

Western blot analysis of PSD-95 on a rat cerebrum lysate.  Lane1: 1:250, lane 2: 1: 500, lane 3: 1:1000 dilution of the mouse anti- PSD-95 antibody.

Purified Mouse Anti- PSD-95

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

Western blot analysis of PSD-95 on a rat cerebrum lysate.  Lane1: 1:250, lane 2: 1: 500, lane 3: 1:1000 dilution of the mouse anti- PSD-95 antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

Product Details
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BD Transduction Laboratories™
PostSynaptic Density-95; SAP90
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat PSD-95 aa. 353-504
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
95 kDa
250 µg/ml
AB_397862
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610495 Rev. 1
Antibody Details
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16/PSD-95

PostSynaptic Density (PSD) refers to a dense region of submembranous cytoskeleton found most prominently in postsynaptic membranes of the CNS.  Possible functions for the PSD include regulation and aggregation of receptors,  structural stabilization of the synaptic junction, and transduction of signals from membrane receptors.  Some of the proteins associated with the PSD are fodrin, tubulin, calmodulin, CaM Kinase II, PSD-95, and PSD-93.  PSD-95 (SAP90) is a protein that interacts with the NMDA receptor NMDAR2B, neuronal NOS  (nNOS or bNOS), and other proteins.  PSD-95 contains one SH3 domain in its carboxy-terminal domain, as well as three conserved repeat regions called GLGF or PDZ domains.  nNOS, which is concentrated in synaptic junctions, also contains a PZD domain.  PSD-95 and nNOS interact via their respective PZD domains, which may mediate the binding of nNOS to skeletal muscle syntrophin.

This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.

610495 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610495 Rev.1
Citations & References
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Development References (5)

  1. Brenman JE, Chao DS, Gee SH,. Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains. Cell. 1996; 84(5):757-767. (Biology). View Reference
  2. Cho KO, Hunt CA, Kennedy MB. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron. 1992; 9(5):929-942. (Biology). View Reference
  3. Fagiolini M, Katagiri H, Miyamoto H. Separable features of visual cortical plasticity revealed by N-methyl-D-aspartate receptor 2A signaling. Proc Natl Acad Sci U S A. 2003; 100(5):2854-2859. (Biology: Western blot). View Reference
  4. Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA. Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain. J Biol Chem. 2002; 277(1):486-491. (Biology: Western blot). View Reference
  5. Mehta S, Wu H, Garner CC, Marshall J. Molecular mechanisms regulating the differential association of kainate receptor subunits with SAP90/PSD-95 and SAP97. J Biol Chem. 2001; 276(19):16092-16099. (Biology: Western blot). View Reference
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610495 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.