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Purified Mouse Anti-PP2A Catalytic α
Purified Mouse Anti-PP2A Catalytic α

Western blot analysis of PP2A Catalytic α on A431 lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of PP2A Catalytic α.

Purified Mouse Anti-PP2A Catalytic α

Catalytic alpha (clone 46) staining on rat brain. Formalin fixed paraffin section without citrate buffer pretreatment. 40X

Western blot analysis of PP2A Catalytic α on A431 lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of PP2A Catalytic α.

Catalytic alpha (clone 46) staining on rat brain. Formalin fixed paraffin section without citrate buffer pretreatment. 40X

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human PP2A aa. 153-309
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
36 kDa
250 µg/ml
AB_397909
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Recommended Assay Procedure:

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610556 Rev. 1
Antibody Details
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46

Protein phosphatases of the type 2A (PP2A) are composed of two regulatory and one catalytic subunit. The different subunits are encoded by multiple genes. PP2A are specific for phosphoserine and phosphothreonine residues of proteins that are involved in the regulation of cellular growth. PP2A binds and dephosphorylates the middle T antigens of polyoma virus. This interaction may regulate the transcriptional activity of the virus. The 36 kDa catalytic subunit is rapidly tyrosine phosphorylated as a result of growth factor stimulation or cell transformation. Tyrosine phosphorylation of the catalytic subunit may inactivate the phosphatase activity of PP2A.

610556 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610556 Rev.1
Citations & References
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Development References (5)

  1. Chen J, Parsons S, Brautigan DL. Tyrosine phosphorylation of protein phosphatase 2A in response to growth stimulation and v-src transformation of fibroblasts. J Biol Chem. 1994; 269(11):7957-7962. (Biology). View Reference
  2. Efendiev R, Yudowski GA, Zwiller J, et al. Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis. J Biol Chem. 2002; 277(46):44108-44114. (Clone-specific: Immunofluorescence, Western blot). View Reference
  3. Nunbhakdi-Craig V, Machleidt T, Ogris E, Bellotto D, White CL 3rd, Sontag E. Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex. J Cell Biol. 2002; 158(5):967-978. (Clone-specific: Western blot). View Reference
  4. Reiken S, Gaburjakova M, Guatimosim S. Protein kinase A phosphorylation of the cardiac calcium release channel (ryanodine receptor) in normal and failing hearts. Role of phosphatases and response to isoproterenol. J Biol Chem. 2003; 278(1):444-453. (Clone-specific: Western blot). View Reference
  5. Yang J, Fan GH, Wadzinski BE, Sakurai H, Richmond A. Protein phosphatase 2A interacts with and directly dephosphorylates RelA. J Biol Chem. 2002; 276(51):47828-47833. (Clone-specific: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610556 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.