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Purified Mouse Anti-p70 [S6K]
Purified Mouse Anti-p70 [S6K]
Western blot analysis of p70 [S6K] on a rat liver lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1: 1000 dilution of the mouse anti-p70[S6K] antibody.
Purified Mouse Anti-p70 [S6K]
Immunofluorescence staining of RSV-3T3 cells.
Western blot analysis of p70 [S6K] on a rat liver lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1: 1000 dilution of the mouse anti-p70[S6K] antibody.
Immunofluorescence staining of RSV-3T3 cells.
Product Details
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BD Transduction Laboratories™
p70 S6 Kinase
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Rat p70 [S6K] aa. 2-121
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
60-70 kDa
250 µg/ml
AB_398791
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611260 Rev. 1
Antibody Details
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16/p70[s6k]

Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational components, such as ribosomal protein S6.  p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6 phosphorylation, belongs to a family of at least six S6 kinases.  p70 [S6K] and p85 [S6K] are two isoforms of the same kinase.  Although they are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence.  These kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic stimulation.  Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389.  p70 [S6K] associates with PDK-1 and PKCζ to form a possible PI3-K signaling complex.  The immunosuppressive agent rapamycin forms an intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme activation.  Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and proliferative responses.  p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting.

611260 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611260 Rev.1
Citations & References
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Development References (4)

  1. Brennan P, Babbage JW, Thomas G, Cantrell D. p70(s6k) integrates phosphatidylinositol 3-kinase and rapamycin-regulated signals for E2F regulation in T lymphocytes. Mol Biol Cell. 1999; 19(7):4729-4738. (Biology). View Reference
  2. Johanson SO, Naccache PA, Crouch MF. A p85 subunit-independent p110alpha PI 3-kinase colocalizes with p70 S6 kinase on actin stress fibers and regulates thrombin-stimulated stress fiber formation in swiss 3T3 cells. Exp Cell Res. 1999; 10(1):223-233. (Biology). View Reference
  3. Ming XF, Burgering BM, Wennstrom S. Activation of p70/p85 S6 kinase by a pathway independent of p21ras. Nature. 1994; 371(6496):426-429. (Biology). View Reference
  4. Romanelli A, Martin KA, Toker A, Blenis J. p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex. Mol Cell Biol. 1999; 19(4):2921-2928. (Biology). View Reference
View All (4) View Less
611260 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.