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Purified Mouse Anti-OPA1
Purified Mouse Anti-OPA1
Western blot analysis of OPA1 on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:500, lane 2: 1000, lane 3: 1: 2000 dilution of the mouse anti- OPA1 antibody.
Purified Mouse Anti-OPA1
Immunofluorescence staining of COS-7 cells (African Green Monkey SV40 transformed kidney cells; ATCC CRL-1651).
Western blot analysis of OPA1 on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:500, lane 2: 1000, lane 3: 1: 2000 dilution of the mouse anti- OPA1 antibody.
Immunofluorescence staining of COS-7 cells (African Green Monkey SV40 transformed kidney cells; ATCC CRL-1651).
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse,Rat,Dog,Chicken (Tested in Development)
Mouse IgG1
Human OPA1 aa. 708-830
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
80-100 kDa
250 µg/ml
AB_399889
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612607 Rev. 1
Antibody Details
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18/OPA1

Three major GTP-binding protein families include trimeric and low molecular weight G-proteins, as well as a family of large proteins homologous to dynamin. The dynamin family contains proteins with diverse structure and function, but highly homologous N-terminal GTPase domains. A subgroup of the dynamin G-protein-binding family includes the mitochondrial proteins Drp1/Dnm1, Mgm1, and OPA1. The latter protein is mutated in dominant optic atrophy, a disease that involves loss of visual acuity and atrophy of the optic nerve. OPA1 is expressed in heart, brain, liver, and kidney. The sequence of OPA1 includes an N-terminal region that contains a mitochondrial targeting domain and three GTP-binding motifs. The overexpression of OPA1 in Cos-7 cells shows co-localization with cytochrome c in mitochondria, and leads to alterations in mitochondrial morphology from a characteristic tubuluar shape to a vesicular pattern. Thus, OPA1 may have roles in mitochondrial biogenesis that are critical for normal cell function.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612607 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612607 Rev.1
Citations & References
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Development References (3)

  1. Alexander C, Votruba M, Pesch UE, et al. OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28. Nat Genet. 2000; 26(2):211-215. (Biology). View Reference
  2. Delettre C, Lenaers G, Griffoin JM, et al. Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy. Nat Genet. 2000; 26(2):207-210. (Biology). View Reference
  3. Misaka T, Miyashita T, Kubo Y. Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology. J Biol Chem. 2002; 277(18):15834-15842. (Biology). View Reference
612607 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.