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Purified Mouse Anti-Mouse iNOS
Purified Mouse Anti-Mouse iNOS

Western blot analysis of iNOS on a lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours. Lane 1: 1:2000, lane 2: 1:4000, lane 3: 1:8000 dilution of the mouse anti-iNOS/NOS Type II antibody.

Purified Mouse Anti-Mouse iNOS

Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS.

Western blot analysis of iNOS on a lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours. Lane 1: 1:2000, lane 2: 1:4000, lane 3: 1:8000 dilution of the mouse anti-iNOS/NOS Type II antibody.

Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS.

Product Details
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BD Transduction Laboratories™
NOS Type II
Mouse (QC Testing)
Mouse IgG2a, κ
Mouse iNOS aa. 961-1144
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
130 kDa
250 µg/ml
AB_397808
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Antibody Details
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54/iNOS

Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-γ, TNF-α, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFκB. Data indicates that TGF-β affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. This antibody has been reported to cross-react with nNOS and eNOS.

Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
Citations & References
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Development References (5)

  1. Resta TC, O'Donaughy TL, Earley S, Chicoine LG, Walker BR. Unaltered vasoconstrictor responsiveness after iNOS inhibition in lungs from chronically hypoxic rats. Am J Physiol. 1999; 276(1 Pt 1):L122-L130. (Biology: Western blot). View Reference
  2. Vodovotz Y, Bogdan C, Paik J, Xie QW, Nathan C. Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta. J Exp Med. 1993; 178(2):605-613. (Biology). View Reference
  3. Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). View Reference
  4. Zadeh MS, Kolb JP, Geromin D. Regulation of ICAM-1/CD54 expression on human endothelial cells by hydrogen peroxide involves inducible NO synthase. J Leukoc Biol. 2000; 67(3):327-334. (Biology: Flow cytometry). View Reference
  5. Zhao H, Dugas N, Mathiot C, et al. B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity. Blood. 1998; 92(3):1031-1043. (Biology: Flow cytometry, Immunofluorescence, Western blot). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.