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Purified Mouse Anti-mDia1
Purified Mouse Anti-mDia1

Western blot analysis of mDia1 on a RSV-3T3 cell lysate.  Lane of 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-mDia1 antibody.

Purified Mouse Anti-mDia1

Immunofluorescence staining of FHs cells (Normal human fetal lung fibroblasts; ATCC HTB-157).

 

Western blot analysis of mDia1 on a RSV-3T3 cell lysate.  Lane of 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-mDia1 antibody.

Immunofluorescence staining of FHs cells (Normal human fetal lung fibroblasts; ATCC HTB-157).

 

Product Details
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BD Transduction Laboratories™
p140mDia; Drf1
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG1
Mouse p140mDia aa. 41-153
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
140 kDa
250 µg/ml
AB_398167
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610848 Rev. 1
Antibody Details
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51/mDia1

GTPases like Ras, Rho, cdc42Hs, and Rac modulate a multitude of cellular functions like cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis. mDia1, also known as p140mDia and Drf1, is the mammalian homologue of Drosophila's diaphanous, a protein essential for cytokinesis. The 1255 amino acid mDia1 is widely expressed, with a Rho binding domain at the NH2-terminal region, a central polyproline region, and an FH2 domain. This protein also shares homology with the yeast Bn1p essential for budding and the mouse formin necessary for proper limb development. mDia1 binds to GTP-Rho and to the actin-binding protein profilin-all three proteins are co-localized at the lamellipodia in cultured cells. The overexpression of mDia1 promoted the formation of actin filaments, implicating this protein in cell motility events regulated by Rho. mDia1 was also found consistently mutated in familial deafness.

610848 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610848 Rev.1
Citations & References
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Development References (3)

  1. Li F, Higgs HN. The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition. Curr Biol. 2003; 13(15):1335-1340. (Biology). View Reference
  2. Pennisi E. The architecture of hearing. Science. 1997; 278(5341):1223-1224. (Biology). View Reference
  3. Watanabe N, Madaule P, Reid T. p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin. EMBO J. 1997; 16(11):3044-3056. (Biology). View Reference
610848 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.