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Western blot analysis ErbB2 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human ErbB2 antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
BD Transduction Laboratories™ Purified Mouse Anti-Human ErbB2
BD Transduction Laboratories™ Purified Mouse Anti-Human ErbB2
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
ErbB2 (Neu or Her-2) is a member of the erbB family of growth factor receptors. These factors possess constitutive tyrosine kinase activity and are commonly overexpressed in breast and ovarian carcinomas. While other erbB family members' ligands, such a EGF and NDF, are well characterized, a natural ligand for erbB2 remains unknown. ErbB2 forms heterodimers with erbB1/EGFR, erbB3, and erbB4, and can modulate their ligand affinities. Thus, erbB2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB2 may act as a signaling subunit for other receptors rather than a true growth factor receptor. Due to high sequence homology, this antibody may cross-react with the 180 kDa EGFR.
Development References (5)
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Dillon C, Creer A, Kerr K, Kumin A, Dickson C. Basolateral targeting of ERBB2 is dependent on a novel bipartite juxtamembrane sorting signal but independent of the C-terminal ERBIN-binding domain. Mol Cell Biol. 2002; 22(18):6553-6563. (Biology: Western blot). View Reference
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Graus-Porta D, Beerli RR, Hynes NE. Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling. Mol Cell Biol. 1995; 15(3):1182-1191. (Biology). View Reference
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Piechocki MP, Pilon SA, Wei WZ. Complementary antitumor immunity induced by plasmid DNA encoding secreted and cytoplasmic human ErbB-2. J Immunol. 2001; 167(6):3367-3374. (Biology: Flow cytometry, Immunofluorescence). View Reference
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Szollosi J, Balazs M, Feuerstein BG, Benz CC, Waldman FM. ERBB-2 (HER2/neu) gene copy number, p185HER-2 overexpression, and intratumor heterogeneity in human breast cancer. Cancer Res. 1995; 55(22):5400-5407. (Biology). View Reference
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Xu W, Mimnaugh E, Rosser MF. Sensitivity of mature Erbb2 to geldanamycin is conferred by its kinase domain and is mediated by the chaperone protein Hsp90. J Biol Chem. 2001; 276(5):3702-3708. (Biology: Western blot). View Reference
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