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Purified Mouse Anti-Human CLA-1
Purified Mouse Anti-Human CLA-1

Western blot analysis of CLA-1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1: 500,  lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human CLA-1 antibody.

Purified Mouse Anti-Human CLA-1

Immunofluorescence staining of human endothelial cells.

Western blot analysis of CLA-1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1: 500,  lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human CLA-1 antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
CD36 and LIMPII Analogous-1
Human (QC Testing)
Mouse IgG1
Human CLA-1 aa. 104-294
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
80 kDa
250 µg/ml
AB_2269860
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610882 Rev. 1
Antibody Details
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25/CLA-1

CLA-1 (CD36 and LIMPII Analogous-1) is member of a novel gene family that includes CD36, LIMPII, and SR-BI. CD36 is a cell surface glycoprotein that binds to collagen type I and thrombospondin. LIMPII (lysosomal integral membrane protein II), as its name suggests, is expressed on the membrane of lysosomes. SR-BI (scavenger receptor type B class I) is involved in the selective uptake of cholesterol esters. These proteins include two membrane-anchoring regions, two short cytoplasmic tails, and a large extracellular/luminal domain. CLA-1 mRNA is detected in a wide range of tissues including adrenal glands, liver, and testis. Its expression and similarity with other family members suggest it may play a role in HDL metabolism. However, identification of the CLA-1 receptor on monocytes indicates additional CLA-1 functions in leukocytes.

610882 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610882 Rev.1
Citations & References
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Development References (4)

  1. Acton S, Rigotti A, Landschulz KT, Xu S, Hobbs HH, Krieger M. Identification of scavenger receptor SR-BI as a high density lipoprotein receptor. Science. 1996; 271(5248):518-520. (Biology). View Reference
  2. Calvo D, Vega MA. Identification, primary structure, and distribution of CLA-1, a novel member of the CD36/LIMPII gene family. J Biol Chem. 1993; 268(25):18929-18935. (Biology). View Reference
  3. Kozarsky KF, Donahee MH, Rigotti A, Iqbal SN, Edelman ER, Krieger M. Overexpression of the HDL receptor SR-BI alters plasma HDL and bile cholesterol levels. Science. 1997; 387(6631):414-417. (Biology). View Reference
  4. Lasley RD, Narayan P, Uittenbogaard A, Smart EJ. Activated cardiac adenosine A(1) receptors translocate out of caveolae. J Biol Chem. 2000; 275(6):4417-4421. (Biology: Western blot). View Reference
View All (4) View Less
610882 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.