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Purified Mouse Anti- GST-π
Purified Mouse Anti- GST-π

Western blot analysis of GST-π on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- GST-π antibody.

Purified Mouse Anti- GST-π

Immunofluorescence staining of human fibroblasts.

Western blot analysis of GST-π on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- GST-π antibody.

Immunofluorescence staining of human fibroblasts.

Product Details
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BD Transduction Laboratories™
Glutathione-S-Transferase-π
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human GST-π aa. 5-210
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
23 kDa
250 µg/ml
AB_398042
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610718 Rev. 1
Antibody Details
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3/GST-π

Glutathione-S-Transferases (GSTs) are a family of dimeric proteins that catalyze the S-conjugation of glutathione with many compounds for detoxification. The GSTs are categorized into four classes based on their biochemical and structural properties: α, µ, π, and θ.  GST-π is of particular interest because it is over-expressed in many tumors, but is either absent or expressed at low levels in the corresponding normal tissues. This high expression of GST-π is associated with malignant transformation and correlates with a decrease in GST activity.  Three different GST-π proteins have been isolated and designated as hGSTP1*A, hGSTP1*B, and hGSTP1*C.  These isoforms are nearly identical, varying by only one to two amino acids.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610718 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610718 Rev.1
Citations & References
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Development References (3)

  1. Ali-Osman F, Akande O, Antoun G, Mao JX, Buolamwini J. Molecular cloning, characterization, and expression in Escherichia coli of full-length cDNAs of three human glutathione S-transferase Pi gene variants. Evidence for differential catalytic activity of the encoded proteins. J Biol Chem. 1987; 272(15):10004-10012. (Biology). View Reference
  2. Kano T, Sakai M, Muramatsu M. Structure and expression of a human class pi glutathione S-transferase messenger RNA. Cancer Res. 1987; 47(21):5626-5630. (Biology). View Reference
  3. Zhou T, Evans AA, London WT. Glutathione S-transferase expression in hepatitis B virus-associated human hepatocellular carcinogenesis. Cancer Res. 1997; 57(13):2749-2753. (Biology). View Reference
610718 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.