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Purified Mouse Anti-GOK/Stim1
Product Details
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BD Transduction Laboratories™
Stim1
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG2a
Human GOK aa. 25-139
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
84 kDa
250 µg/ml
AB_398267
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610954 Rev. 1
Antibody Details
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44/GOK

The human chromosomal region 11p15 has undergone intense analysis because of its association with various malignancies. In particular, the band 11p15.5 contains genes associated with Wilms tumor, Beckwith-Weidemann syndrome, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. One such gene, GOK (Stim 1), was identified near the 5' end of the ribonucleotide reductase subunit 1 gene. Examination of the GOK primary amino acid sequence indicates that it is a typical transmembrane protein with an extracellular N-terminal domain and a cytosolic C-terminal domain. The protein is highly hydrophobic with only a short region of hydrophobicity that likely represents the transmembrane region. The C-terminal portion of GOK shares some small regions of homology with myosin (20% identity). This region of GOK consists of α-helices and is thought to adopt a coiled-coil conformation. Although GOK expression has no effect on the growth of certain breast cancer cell lines, it induces death in rhabdomyosarcoma cells. Thus, it is thought to be a recessive tumor suppressor in muscle cells, possibly by functioning as a receptor connected to an apoptotic signaling pathway.

610954 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610954 Rev.1
Citations & References
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Development References (4)

  1. Hu RJ, Lee MP, Connors TD. A 2.5-Mb transcript map of a tumor-suppressing subchromosomal transferable fragment from 11p15.5, and isolation and sequence analysis of three novel genes. Genomics. 1997; 46(1):9-17. (Biology). View Reference
  2. Overall ML, Parker NJ, Scarcella DL, Smith PJ, Dziadek M. Murine Stim1 maps to distal chromosome 7 and is not imprinted. Mamm Genome. 1998; 9(8):657-659. (Biology). View Reference
  3. Parker NJ, Begley CG, Smith PJ, Fox RM. Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5. Genomics. 1996; 37(2):253-256. (Biology). View Reference
  4. Sabbioni S, Barbanti-Brodano G, Croce CM, Negrini M. GOK: a gene at 11p15 involved in rhabdomyosarcoma and rhabdoid tumor development. Cancer Res. 1997; 57(20):4493-4497. (Biology). View Reference
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610954 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.