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Purified Mouse Anti-Glucocorticoid Receptor
Purified Mouse Anti-Glucocorticoid Receptor

Western blot analysis of glucocorticoid receptor on HeLa cell lysate (left). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-glucocorticoid receptor.

Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well.  After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glucocorticoid Receptor antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software. This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

Western blot analysis of glucocorticoid receptor on HeLa cell lysate (left). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-glucocorticoid receptor.

Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well.  After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glucocorticoid Receptor antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software. This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Glucocorticoid Receptor α aa. 176-289
Western blot (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development)
94 kDa
250 µg/ml
AB_398757
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Triton is a trademark of the Dow Chemical Company.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611227 Rev. 2
Antibody Details
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41/Glucocorticoid Receptor

Steroid hormone receptors are hormone activated transcriptional regulators that influence genes required for embryonic development and adult homeostasis. One member of the steroid hormone family is the glucocorticoid receptor. It contains AF1 and AF2 transactivation domains, a DNA binding domain, and ligand binding domain. Ligand bound glucocorticoid receptors dimerize at specific palindromic sequences called glucocorticoid response elements (GREs) in the cis-regulatory region of target genes. Both AF1 and AF2 may be important for initiation or regulation of transcription by interacting with components of the initiation complex or other intermediary factors. In addition to transactivation, glucocorticoid receptors may also regulate transcription through transrepression of target genes. Although mechanisms of transrepression are not completely understood; DNA binding alone, DNA binding plus interaction with other transcription factors, or protein-protein interaction without DNA binding are mechanisms that have been implicated. Thus, glucocorticoid receptors function in the regulation of specific genes that are essential for human development and homeostasis.

611227 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611227 Rev.2
Citations & References
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Development References (3)

  1. Beato M, Herrlich P, Schutz G. Steroid hormone receptors: many actors in search of a plot. Cell. 1995; 83(6):851-857. (Biology). View Reference
  2. Hollenberg SM, Weinberger C, Ong ES, et al. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature. 1985; 318(6047):635-641. (Biology). View Reference
  3. Reichardt HM, Kaestner KH, Tuckermann J, et al. DNA binding of the glucocorticoid receptor is not essential for survival. Cell. 1998; 93(4):531-541. (Biology). View Reference
611227 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.