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Purified Mouse Anti-Flotillin-2
Purified Mouse Anti-Flotillin-2

Western blot analysis of Flotillin-2 on human endothelial cell lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Flotillin-2.

Purified Mouse Anti-Flotillin-2

Immunofluorescent staining of WI38 cells.

Western blot analysis of Flotillin-2 on human endothelial cell lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Flotillin-2.

Immunofluorescent staining of WI38 cells.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human ESA aa. 122-379
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
42 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot: Please refer to

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to for technical protocols.
610384 Rev. 2
Antibody Details
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Flotillin-2/ESA (epidermal surface antigen) plays a role in intercellular epidermal adhesion. Epidermal surface proteins have an important role in normal epidermal maintenance and function. In addition, they are involved in various autoimmune diseases in which auto-antibodies bind to normal epidermal cell surface proteins. This binding causes cell disadherence that leads to blister formation. Such antibodies have been utilized to identify new proteins involved in epidermal cell adhesion. One of these antibodies, ECS-1, was used to isolate Flotillin-2/ESA. The single copy ESA gene is located on the long arm of human chromosome 17, in the region containing the gene for neurofibromatosis 1 (NF1). ESA mRNA is expressed in cultured keratinocytes, melanocytes, fibroblasts, and carcinoma and melanoma cell lines. Flotillin-2/ESA may play a critical role in epidermal structure and maintenance.

610384 Rev. 2
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610384 Rev.2
Citations & References
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Development References (5)

  1. Nebl T, Pestonjamasp KN, Leszyk JD, Crowley JL, Oh SW, Luna EJ. Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes. J Biol Chem. 2002; 277(45):43399-43409. (Clone-specific: Western blot). View Reference
  2. Rybin VO, Xu X, Lisanti MP, Steinberg SF. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 2000; 275(52):41447-41457. (Clone-specific: Western blot). View Reference
  3. Salzer U, Hinterdorfer P, Hunger U, Borken C, Prohaska R. Ca(++)-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin. Blood. 2002; 99(7):2569-2577. (Clone-specific: Western blot). View Reference
  4. Schroeder WT, Stewart-Galetka S, Mandavilli S, Parry DA, Goldsmith L, Duvic M. Cloning and characterization of a novel epidermal cell surface antigen (ESA). J Biol Chem. 1994; 269(31):19983-19991. (Biology). View Reference
  5. Stuermer CA, Lang DM, Kirsch F, Wiechers M, Deininger SO, Plattner H. Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2. Mol Biol Cell. 2001; 12(10):3031-3045. (Clone-specific: Electron microscopy, Immunofluorescence, Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610384 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.