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      Purified NA/LE Rat Anti-Mouse IL-4
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG2b
      Recombinant Mouse IL-4
      ELISA (Routinely Tested), Neutralization (Tested During Development)
      1.0 mg/ml
      AB_395360
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      ELISA:  The purified format of BVD4-1D11 antibody (Cat. No. 554387) can be useful as a capture antibody in a sandwich ELISA for measuring mouse IL-4.  Purified BVD4-1D11 antibody can be paired with the biotinylated BVD6-24G2 antibody (Cat. No. 554390) as the detecting antibody, with recombinant mouse IL-4 (Cat. No. 550067) as the standard. For testing mouse IL-4 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Mouse IL-4 Set (Cat. No. 555232).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554385 Rev. 2
      Antibody Details
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      BVD4-1D11

      The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4.

      554385 Rev. 2
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554385 Rev.2
      Citations & References
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      Development References (6)

      1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA). View Reference
      2. Finkelman FD, Madden KB, Morris SC, et al. Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes. J Immunol. 1993; 151(3):1235-1244. (Clone-specific: ELISA). View Reference
      3. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Clone-specific: ELISA). View Reference
      4. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). View Reference
      5. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA). View Reference
      6. Schlaak JF, Schmitt E, Hüls C, Meyer zum Büschenfelde KH, Fleischer B. A sensitive and specific bioassay for the detection of human interleukin-10. J Immunol Methods. 1994; 168(1):49-54. (Methodology: Bioassay). View Reference
      View All (6) View Less
      554385 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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