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Human IL-8 ELISA Kit II

BD OptEIA™ Human IL-8 ELISA Kit II

Product Details
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Human (QC Testing)
ELISA (Routinely Tested)

Preparation And Storage

Store unopened reagents at 2-8°C. do not use reagents after expiration date, or if turbidity is evident. Before use, bring all reagents to room temperature (18-25°C). Immediately after use, return to proper storage conditions. Lyophilized standards are stable until expiration date. After reconstitution, immediately aliquot standard stock in polypropylene vials at 50 µl per vial anf freeze at -80°C for up to 6 months. If necessary, store at 2-8°C for up to 8 hours prior to aliquotting/freezing. Do not leave reconstituted standard at room temperature.
550999 Rev. 1
Citations & References
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Development References (17)

  1. Bazzoni F, Cassatella MA, Rossi F, et al. Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8. J Exp Med. 1991; 173:771-774. (Biology).
  2. Callard, RE, and AJH Gearing, ed. The Cytokine Factsbook. London: Academic Press; 1994.
  3. Elner, VM, et al. Neutrophil chemotactic factor (IL-8) gene expression by cytokine-treated retinal pigment epithelial cells. Am J Pathol. 1990; 136:745-750. (Biology).
  4. Gimbrone MA, Obin MS, Brock AF, et al. Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions. Science. 1989; 246:1601-1603. (Biology).
  5. Gregory, H, et al. Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP). Biochem Biophys Res Comm. 1988; 151:883. (Biology).
  6. Luger, TA, et al. Chemotactic properties of partially purified human epidermal cellderived thymocyte-activating factor (ETAF) for polymorphonuclear and mononuclear cells. J Immunol. 1983; 131:816-820. (Biology).
  7. Matsushima K, Morishita K, Yoshimura T, et al. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by Interleukin 1 and tumor necrosis factor. J Exp Med. 1988; 167:1883-1893. (Biology).
  8. Sauder DN, Mounessa NL, Katz SI, Dinarello CA, Gallin JI. Chemotactic cytokines: the role of leukocytic pyrogen and epidermal cell thymocyte activating factor in neutrophil chemotaxis. J Immunol. 1984; 132(2):828-832. (Biology). View Reference
  9. Schroder, JM, et al. J Immunol. 1987; 139:8288. (Biology).
  10. Streiter, RM, et al. Biochem Biophys Res Comm. 1990; 173:725. (Biology).
  11. Streiter, RM, et al. Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-α, LPS, and IL-1 β. Science. 1989; 243:1467-1469. (Biology).
  12. Takahashi, GW, et al. Effect of granulocyte-macrophage colony-stimulating factor and interleukin-3 on interleukin-8 production by human neutrophils and monocytes. Blood. 1993; 81:357-364. (Biology).
  13. Van Damme, J, et al. A novel, NH2-terminal sequence-characterized human monokine possessing neutrophil chemotactic, skin-reactive, and granulocytosis-promoting activity. J Exp Med. 1988; 167:1364-1376. (Biology).
  14. Van Damme, J, et al. Homogeneous interferon-inducing factor is related to endogenous pyrogen and interleukin-1. Nature. 1985; 314:266. (Biology).
  15. Van Damme, J, et al. The chemotactic activity for granulocytes produced by virally infected fibroblasts is identical to monocyte-derived interleukin 8. Eur J Immunol. 1989; 19:1189-1194. (Biology).
  16. Walz, A, et al. Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes. Biochem Biophys Res Commun. 1987; 149:755-761. (Biology).
  17. Yoshimura, T, et al. Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines. Proc Natl Acad Sci. 1987; 84:9233-9237. (Biology).
View All (17) View Less
550999 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.