Preparation And Storage
Recommended Assay Procedures
In a quantitative sandwich ELISA for measuring IgE, this antibody may be used at concentration of 1:1000 dilution and be paired with purified anti-human IgE clone G7-18 (Cat. No. 555894).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The G7-26 monoclonal antibody specifically recognizes the constant region of human Immunoglobulin E (IgE). It does not crossreact with other immunoglobulin heavy chain isotypes. IgE exists in a transmembrane form that is expressed by B lymphocytes and serves as an antigen receptor. Soluble IgE is produced and secreted by activated B cells and plasma cells. IgE may bind through its constant region to cell surface receptors, such as the high-affinity (FcεRI) or low-affinity (FcεRII/CD23) receptors for IgE. FcεRI is expressed on mast cells, basophils, and at lower levels, on dendritic cells and monocytes. FcεRII/CD23 is expressed by B cells and by some other cell types including T cells, monocytes, eosinophils, neutrophils, follicular dendritic cells, and Langerhans cells. Crosslinking of Fc receptor-bound IgE antibodies by multivalent antigens or allergens can induce phagocytosis or the cellular release of inflammatory mediators. Although IgE can provide immune protection against pathogenic parasites, it may also play a central role in a variety of allergic disorders.
Development References (3)
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Brinkley M. A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Bioconjug Chem. 1992; 3(1):2-13. (Biology). View Reference
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Mattson G, Conklin E, Desai S, Nielander G, Savage MD, Morgensen S. A practical approach to crosslinking. Mol Biol Rep. 1993; 17(3):167-183. (Biology). View Reference
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Weston PD, Devries JA, Wrigglesworth R. Conjugation of enzymes to immunoglobulins using dimaleimides. Biochim Biophys Acta. 1980; 612(1):40-90. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
