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Multicolor flow cytometric analysis of Pax5 expression in human and mouse lymphocytes. Human peripheral blood mononuclear cells (PBMC) and BALB/c mouse splenocytes were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574) and then stained with either PE Rat Anti-Mouse Pax5 antibody (Cat. No. 562688) or PE rat IgG2a, κ Isotype Control (Cat. No. 554689). The PBMC were counterstained with BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366) and APC Mouse Anti-Human CD21 (Cat. No. 559867) antibodies. Mouse splenocytes were counterstained with PerCP-Cy™5.5 Rat Anti-Mouse CD45R/B220 (Cat. No. 552771) and FITC Hamster Anti-Mouse CD3e (Cat. No. 553062) antibodies. Upper Panel: Human Peripheral Blood Lymphocytes (PBL): Contour plots from left to right showing the expression of CD21 versus Rat IgG2a κ Isotype Control, CD21 versus Pax5 and CD3 versus Pax5, respectively. Lower Panel: Mouse Splenic Lymphocytes: Contour plots from left to right showing the expression of CD45R/B220 versus Rat IgG2a κ Isotype Control, CD45R/B220 versus PE Pax5 and CD3e versus Pax5, respectively. The flow cytometric data were derived from gated events with the forward and side light-scatter characteristics of intact human (top plots) and mouse (bottom plots) lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
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BD Pharmingen™ PE Rat anti-Pax-5
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
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The 1H9 monoclonal antibody clone specifically binds to human and mouse Paired box protein Pax-5. Pax-5 is a ~50 kDa protein that is also known as B-cell-specific transcription factor and B cell specific activator protein (BSAP). Pax-5 is a member of the paired box (Pax) family of transcription factors. It is expressed in pro-B, pre-B and mature B cells. Through the Groucho family of co-repressors, Pax-5 likely functions as a transcriptional repressor of non-B-lymphoid genes during the B cell commitment process. In the early stages of B cell development, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20 and maintains B cell identity. Pax-5 suppression is involved in the upregulation of Blimp-1 leading to the development of Pax-5-negative plasma cells. Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. Altered forms and expression patterns of Pax-5 have been associated with some lymphoid and nonlymphoid cancers.

Development References (5)
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Adams B, Dorfler P, Aguzzi A, et al. Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev. 1992; 6(9):1589-1607. (Biology). View Reference
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Kallies A, Hasbold J, Fairfax K, et al. Initiation of plasma-cell differentiation is independent of the transcription factor Blimp-1. Immunity. 2007; 26(5):555-566. (Immunogen: Western blot). View Reference
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Klein U, Tu Y, Stolovitzky GA, et al. Transcriptional analysis of the B cell germinal center reaction. Proc Natl Acad Sci U S A. 2003; 100(5):2639-2644. (Biology). View Reference
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O'Brien P, Morin P, Jr., Ouellette RJ, Robichaud GA. The Pax-5 gene: a pluripotent regulator of B-cell differentiation and cancer disease. Cancer Res. 2011; 71(24):7345-7350. (Biology). View Reference
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Zwollo P, Arrieta H, Ede K, Molinder K, Desiderio S, Pollock R. The Pax-5 gene is alternatively spliced during B-cell development. J Biol Chem. 1997; 272(15):10160-10168. (Biology). View Reference
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