PE Mouse Anti-Rat CD106

BD Pharmingen™ PE Mouse Anti-Rat CD106

Name: PE Mouse Anti-Rat CD106
Brand: BD Pharmingen™
SKU: 559229

Clone MR106

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Vcam1; V-CAM 1; VCAM-1; VCAM1B; vascular cell adhesion protein 1

Reactivity: Rat (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Rat VCAM-1-transfected L5178Y

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2214215

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This antibody conjugate has been tested by immunofluorescent staining (≤ 1µg/million cells) with flow cytometric analysis

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

559229 Rev. 5

Antibody Details

MR106

The MR106 monoclonal antibody specifically recognizes CD106, the ~110 kDa transmembrane vascular cell adhesion molecule-1 (VCAM-1). Expression of CD106 on endothelial cells is induced by inflammatory cytokines and in certain pathologic conditions. In the rat bone marrow, CD106 is reportedly expressed on dendritic cells and subsets of the myeloid lineage, but not detectable on peripheral leucocytes. CD106 can reportedly serve as a counter-receptor for VLA-4 (α4β1 integrin) and α4β7 integrin, and it plays an important role in leucocyte extravasation, particularly at sites of inflammation.

559229 Rev. 5

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

559229 Rev.5

Citations & References

Development References (1)

Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology). View Reference

559229 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.