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PE Mouse Anti-Rat CD106
Product Details
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BD Pharmingen™
Vcam1; V-CAM 1; VCAM-1; VCAM1B; vascular cell adhesion protein 1
Rat (QC Testing)
Mouse IgG1, κ
Rat VCAM-1-transfected L5178Y
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This antibody conjugate has been tested by immunofluorescent staining (≤ 1µg/million cells) with flow cytometric analysis

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
559229 Rev. 5
Antibody Details
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The MR106 monoclonal antibody specifically recognizes CD106, the ~110 kDa transmembrane vascular cell adhesion molecule-1 (VCAM-1). Expression of CD106 on endothelial cells is induced by inflammatory cytokines and in certain pathologic conditions. In the rat bone marrow, CD106 is reportedly expressed on dendritic cells and subsets of the myeloid lineage, but not detectable on peripheral leucocytes. CD106 can reportedly serve as a counter-receptor for VLA-4 (α4β1 integrin) and α4β7 integrin, and it plays an important role in leucocyte extravasation, particularly at sites of inflammation.

559229 Rev. 5
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559229 Rev.5
Citations & References
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Development References (1)

  1. Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology). View Reference
559229 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.