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Flow cytometric analysis of CDX-2 expression on human DLD-1 cells. Cells from the human DLD-1 (Colorectal adenocarcinoma, ATCC CCL-221) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and washed with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). The cells were then permeabilized and stained in BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-CDX-2 antibody (Cat. No. 563428; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side scattered-light characteristics of intact cells. This antibody also works with cells permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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BD Pharmingen™ PE Mouse Anti-Human CDX-2
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Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The M39-711 monoclonal antibody reacts with caudal-type homeobox transcription factor 2 (CDX-2). In embryonic development, CDX-2 is first expressed in the outer layer of the morula and later in the trophoblast, but not in embryonic stem cells. Along with Oct3/4, it is involved in the segregation of the trophoectoderm lineage from the inner cell mass. In the adult, CDX-2 expression is limited to the mucosa of the small and large intestines, where it regulates the synthesis of several intestine-specific proteins. Loss of CDX-2 expression is observed in some colorectal carcinomas, while gain of CDX-2 expression has been observed in gastric tumors and acute myeloid leukemias.

Development References (6)
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Hinoi T, Loda M, Fearon ER. Silencing of CDX2 expression in colon cancer via a dominant repression pathway. J Biol Chem. 2003; 278(45):44608-44616. (Biology). View Reference
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Qualtrough D, Hinoi T, Fearon E, Paraskeva C. Expression of CDX2 in normal and neoplastic human colon tissue and during differentiation of an in vitro model system. Gut. 2002; 51:184-190. (Biology). View Reference
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Ralston A, Rossant J. Genetic regulation of stem cell origins in the mouse embryo. Clin Genet. 2005; 68(2):106-112. (Biology). View Reference
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Rawat VP, Cusan M, Deshpande A, et al. Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of (12;13)(p13;q12) acute myeloid leukemia. Proc Natl Acad Sci U S A. 2004; 101(3):817-822. (Biology). View Reference
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Scholl C, Bansal D, Döhner K, et al. The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis. J Clin Invest. 2007; 117(4):865-868. (Biology). View Reference
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Tsukamoto T, Mizoshita T, Tatematsu M. Gastric-and-intestinal mixed-type intestinal metaplasia: aberrant expression of transcription factors and stem cell intestinalization. Gastric Cancer. 2006; 9(3):156-166. (Biology). View Reference
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