Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Denmark (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      PE Mouse Anti-Human CD99
      PE Mouse Anti-Human CD99
      Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
      PE Mouse Anti-Human CD99
      Multiparameter flow cytometric analysis of CD99 expression on human peripheral blood leucocytes populations. Whole blood was stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; Left Plot) or PE Mouse Anti-Human CD99 antibody (Cat. No. 555689; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD99 expression (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
      PE Mouse Anti-Human CD99 PE Mouse Anti-Human CD99
      Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
      Multiparameter flow cytometric analysis of CD99 expression on human peripheral blood leucocytes populations. Whole blood was stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; Left Plot) or PE Mouse Anti-Human CD99 antibody (Cat. No. 555689; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD99 expression (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      E2; MIC2
      Human (QC Testing)
      Mouse IgG2a, κ
      Flow cytometry (Routinely Tested)
      20 µl
      IV N92
      AB_396040
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      Show More blue down arrow Show Less blue up arrow
      555689 Rev. 7
      Antibody Details
      Down Arrow Up Arrow
      TÜ12

      The TÜ12 monoclonal antibody specifically recognizes CD99, also referred to as E2 antigen, a 32 kDa sialoglycoprotein expressed on all leucocyte lineages. The E2 antigen is the MIC2 gene product and is differentially expressed during T- and B-lymphoid and granulocytic development, with higher densities being expressed during early hematopoietic stages. Mature granulocytes express  very little or no CD99. E2 has been shown to be involved in T-cell adhesion processes and is suggested to have a functional role in hematopoietic adhesion pathways.

      555689 Rev. 7
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 561 nm
      496 nm, 566 nm
      576 nm
      555689 Rev.7
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "555689" on CiteAb

      Development References (6)

      1. Aussel C, Bernard G, Breittmayer JP, Pelassy C, Zoccola D, Bernard A. Monoclonal antibodies directed against the E2 protein (MIC2 gene product) induce exposure of phosphatidylserine at the thymocyte cell surface. Biochemistry. 1993; 32(38):10096-10101. (Biology). View Reference
      2. Chang A, Benda PM, Wood BL, Kussick SJ. Lineage-specific identification of nonhematopoietic neoplasms by flow cytometry.. Am J Clin Pathol. 2003; 119(5):643-55. (Clone-specific: Flow cytometry). View Reference
      3. Dworzak MN, Fritsch G, Buchinger P, et al. Flow cytometric assessment of human MIC2 expression in bone marrow, thymus, and peripheral blood. Blood. 1994; 83(2):415-425. (Biology). View Reference
      4. Gelin C, Aubrit F, Phalipon A, et al. The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989; 8(11):3253-3259. (Biology). View Reference
      5. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      6. Uchańska-Ziegler B, Wernet P, Ziegler A. Differentiation of a human myeloid cell line (HL-60) toward granulocyte- and macrophage-like cells: comparison of cell surface antigen expression.. Haematol Blood Transfus. 1983; 28:386-8. (Clone-specific: Immunofluorescence). View Reference
      View All (6) View Less
      555689 Rev. 7

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.