Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reacts with the 130 kDa integrin β1 subunit, CD29, that is expressed following cell activation as a heterodimeric complex with one of nine distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The HUTS-21 β1 epitope is weakly expressed on resting lymphocytes, but is upregulated by TPA, Ca++ ionophore, or another CD29 antibody, TS2/16. This upregulation is Mn++ dependent and not supported by other divalent cations such as Ca++ and/or Mg++. The VLA receptors have a broad cellular distribution and interact with ligands like VCAm-1 and MAdCAM-1 during cell adhesion. They also interact with extra-cellular matrices including collagen, laminin, fibronectin and others. HUTS-21 antibody enhances adherence of TPA-activated lymphocytes to fibronectin.
Development References (5)
-
Cabanas C, Hogg N. Ligand intercellular adhesion molecule 1 has a necessary role in activation of integrin lymphocyte function-associated molecule 1. Proc Natl Acad Sci U S A. 1993; 90(12):5838-5842. (Biology). View Reference
-
Garcia-Gila M, Cabanas C, Garcia-Pardo A. Analysis of the activation state of alpha4beta1 integrin in human B cell lines derived from myeloma, leukemia or lymphoma. FEBS Lett. 1997; 418(3):337-340. (Biology). View Reference
-
Gomez J, Garcia A, R-Borlado L, et al. IL-2 signaling controls actin organization through Rho-like protein family, phosphatidylinositol 3-kinase, and protein kinase C-zeta. J Immunol. 1997; 158(4):1516-1522. (Biology). View Reference
-
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
-
Luque A, Gomez M, Puzon W, Takada Y, Sanchez-Madrid F, Cabanas C. Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem. 1996; 271(19):11067-11075. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
