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BD Pharmingen™ PE Mouse anti-Human B7-H4
Clone MIH43 (RUO)
Flow cytometric analysis of human B7-H4 protein expression on B7-H4-transfected cells. Non-transfected (Left Panel) and B7-H4-transfected (Right Panel) mouse P815 mastocytoma cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse anti-Human B7-H4 (Cat. No. 562507; solid line histogram). Flow cytometric fluorescence histograms showing the expression of B7-H4 (or Ig Isotype staining) on non-transfected or transfected cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of human B7-H4 protein expression on B7-H4-transfected cells. Non-transfected (Left Panel) and B7-H4-transfected (Right Panel) mouse P815 mastocytoma cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse anti-Human B7-H4 (Cat. No. 562507; solid line histogram). Flow cytometric fluorescence histograms showing the expression of B7-H4 (or Ig Isotype staining) on non-transfected or transfected cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of human B7-H4 protein expression on B7-H4-transfected cells. Non-transfected (Left Panel) and B7-H4-transfected (Right Panel) mouse P815 mastocytoma cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse anti-Human B7-H4 (Cat. No. 562507; solid line histogram). Flow cytometric fluorescence histograms showing the expression of B7-H4 (or Ig Isotype staining) on non-transfected or transfected cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of human B7-H4 protein expression on B7-H4-transfected cells. Non-transfected (Left Panel) and B7-H4-transfected (Right Panel) mouse P815 mastocytoma cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse anti-Human B7-H4 (Cat. No. 562507; solid line histogram). Flow cytometric fluorescence histograms showing the expression of B7-H4 (or Ig Isotype staining) on non-transfected or transfected cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
VTCN1; VCTN1; B7 family member, H4; B7H4; B7h.5; B7S1; B7X
Human (QC Testing)
Mouse IgG1, κ
Recombinant Human B7-H4 protein
Flow cytometry (Routinely Tested)
5 µl
AB_11154215
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
562507 Rev. 2
Antibody Details
MIH43
The MIH43 monoclonal antibody specifically binds to human B7-H4 (B7 family member, H4) that is encoded by the VTCN1 (V-set domain containing T cell activation inhibitor 1) gene. B7-H4 is a type I membrane glycoprotein with a calculated molecular weight of 30.89 kDa. It is also known as VCTN1, B7h.5, B7S1 (B7 superfamily member 1) or B7X. B7-H4 is a newly discovered member of the B7 family of costimulatory proteins. B7-H4 is not constitutively expressed on peripheral tissues. Its expression can be induced on macrophages, dendritic cells, B cells and T cells. By binding to its putative receptor, the function of B7-H4 was initially reported to be a negative regulator of T cell activation, proliferation and differentiation related to cytokine production and cytotoxic effector cell functions. B7-H4 is reportedly overexpressed in a variety tumors. B7-H4 expression by tumor macrophages appears to play a role in suppression antigen-specific T cell mediated immunity. Recent studies indicate that B7-H4 can also be a positive regulator of T cell responses. B7-H4 can be involved innate immunity as well, eg, by inhibiting neutrophil expansion.
562507 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
562507 Rev.2
Citations & References
View product citations for antibody "562507" on CiteAb
Development References (4)
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Choi IH, Zhu G, Sica GL, et al. Genomic organization and expression analysis of B7-H4, an immune inhibitory molecule of the B7 family. J Immunol. 2003; 171(9):4650-4654. (Biology). View Reference
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Kryczek I, Zou L, Rodriguez P, et al. B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma. J Exp Med. 2006; 203(4):871-881. (Biology). View Reference
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Quandt D, Fiedler E, Boettcher D, Marsch WCh, Seliger B. B7-h4 expression in human melanoma: its association with patients' survival and antitumor immune response.. Clin Cancer Res. 2011; 17(10):3100-11. (Biology). View Reference
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Sica GL, Choi IH, Zhu G, et al. B7-H4, a molecule of the B7 family, negatively regulates T cell immunity. Immunity. 2003; 18(6):849-861. (Biology). View Reference
562507 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.