The RL388 monoclonal antibody recognizes CD98 which is also known as the 4F2 antigen and Ly-10. CD98 is a disulfide-linked heterodimer comprised of an ~86 kDa single-pass type II heavy chain glycoprotein that associates with a ~39-kDa multi-pass, nonglycosylated light chain which belongs to the L-type amino transporter family. The RL388 antibody specifically binds to an epitope present on the CD98 heavy chain (CD98hc, also known as the 4F2 cell-surface antigen heavy chain or 4F2hc) which is encoded by Slc3a2 [solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2]. CD98 is variably expressed on different cell types including T cells, B cells, and NK cells as well as monocytes, granulocytes, platelets, and fibroblasts. CD98 serves as an activation marker for T and B cells since its expression is upregulated upon cellular activation. CD98 regulates cellular growth, proliferation, and survival through CD98hc interactions with β integrins and by CD98 light chain-mediated amino acid transport.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).