BUV395 Mouse Anti-Human CD217 (IL-17RA)

This product will discontinued starting May 2025 and has been replaced with: Clone W23-251

BD OptiBuild™ BUV395 Mouse Anti-Human CD217 (IL-17RA)

Name: BUV395 Mouse Anti-Human CD217 (IL-17RA)
Brand: BUV395 Mouse Anti-Human CD217 (IL-17RA)
SKU: 747945

Clone 133617 (RUO)

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Product Details

Brand: BD OptiBuild™

Alternative Name: IL17RA; IL-17RA; IL17R; interleukin-17 receptor A; IL-17 receptor A; hIL-17R; CANDF5; IMD51

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG1, κ

Immunogen: Human CD217 (IL-17RA) Recombinant Protein

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Workshop Number: HCDM 2006

RRID: AB_2872406

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.

Companion Products

Human BD Fc Block™ RUO

Size 50 µg Cat No. 564219

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

View Details

747945 Rev. 1

Antibody Details

133617

The 133617 monoclonal antibody specifically recognizes CD217 which is also known as Interleukin-17 receptor A (IL-17R or IL-17RA). CD217 is a ~120 kDa single-pass type I transmembrane glycoprotein that is encoded by IL17RA. CD217 (IL-17RA) belongs to the IL-17 receptor family which also includes IL-17 RB, IL-17RC, IL-17RD, and IL-17RE. CD217 (IL-17RA) has a wide tissue distribution and is variably expressed on granulocytes, monocytes, macrophages, dendritic cells (DCs), T cells, B cells, NK cells, smooth muscle cells, endothelial cells, and epithelial cells. Interleukin-17A (IL-17A) or IL-17F homodimers, or IL-17A:IL-17F heterodimers can bind to and signal through functional IL-17 receptor complexes comprised of CD217 (IL-17RA) and IL-17RC subunits. This leads to the cellular release of proinflammatory cytokines including IL-1, IL-6, and TNF, antimicrobial peptides, and chemokines that attract neutrophils. IL-17E/IL-25 binds to and signals through another functional IL-17 receptor complex comprised of CD217 (IL-17RA) and IL-17RB subunits. IL-17E/IL-25 induces type-2 (Th2-like) immune responses and is implicated in asthma. IL-17C binds to IL-17 receptor complexes comprised of CD217 (IL-17RA) and IL-17RE subunits and can stimulate the production of proinflammatory cytokines and antimicrobial peptides.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

747945 Rev. 1

Format Details

BUV395

The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BUV395

Excitation Source: Ultraviolet 355 nm

Excitation Max: 348 nm

Emission Max: 395 nm

747945 Rev.1

Citations & References

View product citations for antibody "747945" on CiteAb

Development References (5)

Ahn SH, Edwards AK, Singh SS, Young SL, Lessey BA, Tayade C. IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors.. J Immunol. 2015; 195(6):2591-600. (Clone-specific: Flow cytometry). View Reference
Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Hölscher C, Tolosa E. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.. J Immunol. 2009; 183(9):5494-501. (Clone-specific: Flow cytometry). View Reference
Gaffen SL. Structure and signalling in the IL-17 receptor family.. Nat Rev Immunol. 2009; 9(8):556-67. (Biology). View Reference
Yao Z, Spriggs MK, Derry JM, et al. Molecular characterization of the human interleukin (IL)-17 receptor. Cytokine. 1997; 9(11):794-800. (Biology). View Reference
Zola H, Swart B, Banham A, et al. CD molecules 2006--human cell differentiation molecules.. J Immunol Methods. 2007; 319(1-2):1-5. (Clone-specific: Flow cytometry). View Reference

View All (5)

747945 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.