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BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8)
Clone 44C143 (RUO)
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human CD288 (TLR8) expression in human peripheral blood leucocyte populations. Human peripheral blood cells were treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. The leucocytes were permeabilized using BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8) antibody (Cat. No. 566327; Right Plot) at 0.06 µg/test. Data shown on this Technical Data Sheet are not lot specific. Two-parameter flow cytometric contour plots showing the correlated expression of CD288 (TLR8) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
TLR8; Toll-like receptor 8
Human (QC Testing)
Mouse IgG1, κ
Human TLR8 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
HCDM 2006
AB_2739687
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Lyse/Fix Buffer 5X RUO
Size
250 ML
Cat No.
558049
Perm/Wash Buffer RUO
Size
100 ML
Cat No.
554723
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557732
566327 Rev. 1
Antibody Details
44C143
The 44C143 monoclonal antibody specifically binds to CD288 which is also known as the Toll-like receptor 8 (TLR8). CD288 is a type 1 transmembrane glycoprotein that belongs to the Toll-like receptor (TLR) family whose members play fundamental roles in pathogen recognition and activation of inflammatory and immune responses. CD288 is expressed in the endosomal and lysosomal compartments of neutrophils, monocytes, macrophages and dendritic cells. CD288 binds single-stranded microbial RNA and is involved in triggering leucocyte responses against bacteria, and single-stranded or double-stranded RNA viruses. Ligand-bound CD288 triggers the MyD88/NF-κB signal pathway leading to antimicrobial cellular responses including the release of proinflammatory cytokines such as TNF, IL-12, and MIP-1α/CCL3.
566327 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
653 nm
669 nm
566327 Rev.1
Citations & References
View product citations for antibody "566327" on CiteAb
Development References (7)
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Bernard MA, Han X, Inderbitzin S, et al. HIV-derived ssRNA binds to TLR8 to induce inflammation-driven macrophage foam cell formation.. PLoS ONE. 2014; 9(8):e104039. (Biology). View Reference
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Cervantes JL, Dunham-Ems SM, La Vake CJ, et al. Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta.. Proc Natl Acad Sci USA. 2011; 108(9):3683-8. (Biology). View Reference
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Chatillon JF, Hamieh M, Bayeux F, et al. Direct Toll-Like Receptor 8 signaling increases the functional avidity of human CD8+ T lymphocytes generated for adoptive T cell therapy strategies.. Immun Inflamm Dis. 2015; 3(1):1-13. (Biology). View Reference
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Chuang TH, Ulevitch RJ. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw. 2000; 11(3):372-378. (Biology). View Reference
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Du X, Poltorak A, Wei Y, Beutler B. Three novel mammalian toll-like receptors: gene structure, expression, and evolution. Eur Cytokine Netw. 2000; 11(3):362-371. (Biology). View Reference
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Zola H, Swart B, Banham A, et al. CD molecules 2006--human cell differentiation molecules.. J Immunol Methods. 2007; 319(1-2):1-5. (Clone-specific: Flow cytometry). View Reference
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Zola H, Swart B, Nicholson I, Voss E. CD288. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:462-463.
566327 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.