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Rat T Lymphocyte Cocktail

Rat T Lymphocyte Cocktail

(RUO)
Rat T Lymphocyte Cocktail

Three-color analysis of the expression of CD3, CD4, and CD8 on rat splenocytes.  A single-cell suspension of Lewis splenocytes was stained with either Isotype Control Cocktail B (Cat. no. 558508; data not shown) or Rat T Lymphocyte Cocktail (Cat. no. 558493).   The figure on the left represents the CD3 and CD4 profile gating on whole spleen.  The figure on the right represents the CD4 and CD8 profile gating on whole spleen.  Flow cytometry was performed on a BD FACSCalibur™.

Three-color analysis of the expression of CD3, CD4, and CD8 on rat splenocytes.  A single-cell suspension of Lewis splenocytes was stained with either Isotype Control Cocktail B (Cat. no. 558508; data not shown) or Rat T Lymphocyte Cocktail (Cat. no. 558493).   The figure on the left represents the CD3 and CD4 profile gating on whole spleen.  The figure on the right represents the CD4 and CD8 profile gating on whole spleen.  Flow cytometry was performed on a BD FACSCalibur™.

Product Details
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BD Pharmingen™
Flow cytometry (Tested During Development)
RUO
AB_771205


Description

               Cocktail Component                 Clone                Isotype

                     APC anti-Rat CD3                        1F4                        mIgM

                     PE anti-Rat CD4                        OX-35                mIgG2a

                     FITC anti-Rat CD8a                OX-8                mIgG1

The Rat T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify rat T lymphocytes by direct immunofluorescence staining with flow cytometric analysis.  The OX-35 antibody reacts with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (ie, MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells (1,2).  The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32-kDa alpha chain of the CD8 differentiation antigen (1,4).  The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells.  The IF4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes and peripheral T lymphocytes (1,3).

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558493 Rev. 1
Citations & References
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Development References (4)

  1. Brideau RJ, Carter PB, McMaster WR, Mason DW, Williams AF. Two subsets of rat T lymphocytes defined with monoclonal antibodies.. Eur J Immunol. 1980; 10:609-615. (Clone-specific). View Reference
  2. Jefferies WA, Green JR, Williams AF. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985; 162(1):117-127. (Immunogen: Flow cytometry, Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Inhibition). View Reference
  3. Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Clone-specific). View Reference
  4. Torres-Nagel N, Kraus E, Brown MH, et al. Differential thymus dependence of rat CD8 isoform expression.. Eur J Immunol. 1992; 22(11):2841-2848. (Biology). View Reference
558493 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.