The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry. It can be used with mouse and human cells during cell staining with cyanine-containing and cyanine-like tandem fluorochrome-conjugated antibodies.
Integrating the BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer into standard flow cytometry staining protocols is simple – add the reagent to either the cell suspension or antibody cocktail. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is compatible with commonly used buffers such as the BD Horizon™ Brilliant Stain Buffer, BD Pharmingen™ Mouse Fc Block™ and BD Pharmingen™ Human BD Fc Block™
The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer should be used when staining cells with cyanine-containing and cyanine-like tandem fluorochromes such as PE-Cy5, PE-Cy7, APC-Cy7, APC-H7 and PE-CF594. These fluorochromes can contribute to varying levels of background staining on monocytes and lymphocytes. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces non-specific interactions of the fluorochrome with leucocytes, revealing true expression of your target markers.
Flow cytometric analysis of myeloid and lymphoid cells with cyanine-containing tandem fluorochromes.
BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on human leukocytes, while maintaining antibody-specific staining
When staining lysed whole blood with cell surface markers (CD279, CD3, CD19) conjugated to PE-Cy7, there is background staining on monocytes. Addition of the BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer to the staining protocol reduces the nonspecific background staining derived from PE-Cy7 on monocytes but does not affect antibody-specific staining of CD279 (PD-1), CD3 and CD19 staining on lymphocytes.
Flow cytometric analysis of human peripheral blood leukocytes with and without BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer.
BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on mouse leukocytes
Background staining with cyanine-containing and cyanine-like tandem fluorochromes can also be observed in mouse leukocytes. In addition, some cell types express Fcγ receptors that can also contribute to background staining by binding non-specifically to detection antibodies. This is commonly addressed by using reagents that block Fc receptors such as BD Pharmingen™ Mouse Fc Block™. BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer can be used in combination with Fc blocking reagents. Together, they help resolve true positive populations from false negatives derived from dye and Fc receptor-mediated staining.
When staining mouse bone marrow cells with PE-Cy7 antibody conjugates without blocking reagents, both lymphoid and myeloid populations show background staining. Treatment with BD Pharmingen™ Mouse Fc Block™ reduces some of the background staining, but non-specific binding of the fluorochrome is still present. Staining cells in the presence of both BD Pharmingen™ Mouse Fc Block™ and the BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer shows further reduced background across three different antibody isotypes. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on leukocytes, while preserving the staining intensity of antibody-specific binding such as CD11b.
Flow cytometric analysis of mouse bone marrow cells with BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer and BD Pharmingen™ Mouse Fc Block™.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry.